The p53 pathway is involved in the inhibition of cell proliferation observed in 15-LOX-1-overexpressing cells. The p53 pathway is involved in the inhibition of cell proliferation observed in 15-LOX-1-overexpressing cells. A. Total RNAs from the stable cells were isolated and then used to probe the Superarray membrane according to the manufacturer's protocol. Cyclin-dependent kinase inhibitor p21WAF1/CIP1 and MDM2 genes were induced in 15-LOX-1-overexpressing cell lines in both membrane-based microarrays (Human Cell Cycle Gene Array and Human p53 Signaling Pathway Gene Array, Superarray). B. Proteins from two cell lines (HCT-116-Vector and HCT-116-15-LOX-1-20) were prepared and each cell lysate (30 μg) was subject to Western blot analysis. p21WAF1/CIP1, MDM2, NAG-1, and actin antibodies were used. C. Cells were seeded in 96-well plates and cell viability was measured at 24-hour intervals as described in Materials and Methods. Points, mean (n = 8) of a representative experiment; bars, SD. Jong-Sik Kim et al. Mol Cancer Res 2005;3:511-517 ©2005 by American Association for Cancer Research