1. Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis using antibody Lt-4 specific for HTLV-1 Tax to determine whether the HTLV-1 Tax protein was present i...
Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis using antibody Lt-4 specific for HTLV-1 Tax to determine whether the HTLV-1 Tax protein was present in PBMCs from two HAM patients (HAM1 and HAM2). The HTLV-1 Tax protein was detected in cell lysates prepared from both HAM patient's PBMC and MT2 cells (positive control HTLV-1+ lymphoma) but not in PBMC from a healthy individual. B and C, the peptide Tax specific HTL line 9B and peptide Tax reactive HTL clone 25 recognized the lysates prepared from HAM patient's PBMC and the response was inhibited by anti-DR mAb L243 at 10 μg/mL. Results correspond to triplicate samples. Columns without bars had SD <10% the value of the mean. Representative of two experiments that were done with the same samples.
Hiroya Kobayashi et al. Clin Cancer Res 2006;12:
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