1. I3C reduces the level of Cdc25A protein in breast cancer cells.
I3C reduces the level of Cdc25A protein in breast cancer cells. MDA-MB-231 (A), MDA-MB-468 (B), and MCF7 (C) breast cancer cells were treated with 0.1% DMSO alone or with 100 to 200 μmol/L I3C for 24, 48, and 72 h. Cell lysates were prepared and Western blot analysis was done with the indicated antibodies. Cdk6 was reduced only in the I3C-treated MCF7 cells, whereas reduced levels of Cdc25A were observed in all cell types with I3C treatment, which attenuated the dephosphorylation of its downstream Cdk2. Representative data from one experiment are shown (n = 3).
Yongsheng Wu et al. Cancer Prev Res 2010;3:
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