1. Expression data from genes involved in regulation of transit through the cell cycle in response to treatment with E2 and OHT. A.
Expression data from genes involved in regulation of transit through the cell cycle in response to treatment with E2 and OHT. A. Expression data were obtained from treated MCF-7 cells in the compiled data set for genes directly involved in cell cycle, transcription factors involved in inducing entry into the cell cycle, and genes involved in replication and/or maintenance of genomic stability. Data are graphically represented as described in Fig. 1. B. Northern analysis of treated MCF-7 cells for myc, myb, stk15, and cyclin D1 (4.3 and 1.5 kb). Inset, duplicate Northern analysis highlighting the up-regulation of myc in response to E2 and OHT at early time points. Loading was assessed by expression of GAPDH RNA. C. Western analysis of treated MCF-7 cells for cyclin D1 and cyclin A2 protein expression. Loading was assessed by expression of actin (cyclin A2) or by a nonspecific band unresponsive to treatment (cyclin D1).
Leslie C. Hodges et al. Mol Cancer Res 2003;1:
©2003 by American Association for Cancer Research