1. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis.
Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A. Time course of the percentage of cells arrested in the G2-M phase of the cell cycle (▪) and apoptotic cells estimated as the sub-G1 population (▴) based on DNA content analysis by flow cytometry. Cells were treated with 20 μmol/L curcumin. Points, mean (n = 4); bars, SD. B. Caspase-3 activity on curcumin treatment. Cells were treated for 6, 18, or 24 hours with curcumin, fixed, stained with anti-active caspase-3 antibody, and analyzed by flow cytometry. Representative histograms and statistical analysis. Columns, mean (n = 3); bars, SD. C. Caspase-7 cleavage in curcumin-treated cells shown by Western blotting in whole-cell extracts was prepared at indicated time points.
Kamila Wolanin et al. Mol Cancer Res 2006;4:
©2006 by American Association for Cancer Research