PTK6 downregulation suppresses SNAIL and increases E-cadherin expression. PTK6 downregulation suppresses SNAIL and increases E-cadherin expression. A, Western blot analysis for SNAIL, SLUG, ZEB1, and TWIST in PTK6-downregulated MDA-MB-231 and MMTV-myc cells. B, Western blot analysis for E-cadherin in MDA-MB-231 and MMTV-myc cells transfected with SNAIL siRNA or SNAIL shRNA. C, Western blot analysis for SNAIL in MCF10A, MDA-MB-231, or BT549 cells overexpressing pBabe vector control, CA-PTK6, or KD-PTK6. For BT549 cells, nuclear lysate was used. D, qRT-PCR analysis for SNAI1 mRNA transcripts in PTK6-downregulated MDA-MB-231 and MMTV-myc cells. Bar graphs are shown as mean ± SEM. All experiments were repeated at least three times. Koichi Ito et al. Cancer Res 2016;76:4406-4417 ©2016 by American Association for Cancer Research