1. Increase in beige/brown adipocyte characteristics in the xenografts from additional breast cancer cell lines.
Increase in beige/brown adipocyte characteristics in the xenografts from additional breast cancer cell lines. Breast cancer cells (2 × 106 cells), purchased from ATCC, were propagated in their respective media, mixed with Matrigel, and implanted subcutaneously (posterior, dorsolateral) in nude mice (n = 5 mice per cell line). A–C, hematoxylin/eosin-stained picture of xenograft sections obtained from MDA-MB-468 cells. A representative picture at low (A) and high magnifications (B and C) is shown. D and E, quantitative real-time PCR analysis using human- and mouse-specific primers in xenografts (5 weeks) from MDA-MB-468 cells. Relative expression levels in the xenografts were compared either with the cells before implantation (human-specific primers) or with mouse tissue at the site of implantation (mouse-specific primers). Data are shown as mean ± SD, (n = 4). F, MDA-MB-468 cells (2 × 106) were mixed with Matrigel and subcutaneously implanted in mice. Mice were killed at different time points and tissue lysates from excised tumors were analyzed by Western blot analysis for different beige/brown-specific proteins. (n = 3 mice per time point). Pooled samples from each time point were analyzed by Western blot analysis. G and H, representative bright field picture of xenograft sections obtained from MDA-MB-231 cells, toluidine blue stain. I and J, quantitative real-time PCR analysis of xenografts obtained after 5 week of implantation of MDA-MB-231 cells compared either with the cells before implantation (human-specific primers) or with mouse tissue at the site of implantation (mouse-specific primers). Data are shown as mean ± SD, (n = 4). K, MDA-MB-231 cells (2 × 106) were mixed with Matrigel and subcutaneously implanted in mice. Mice were killed at different time points and tissue lysates from excised tumors were analyzed by Western blot analysis for different beige/brown–specific proteins. (n = 3 mice per time point). Pooled samples from each time point were analyzed by Western blot analysis.
Rajan Singh et al. Mol Cancer Res 2016;14:78-92
©2016 by American Association for Cancer Research