Martin Depner, PhD, Markus J. Ege, MD, Michael J

Slides:

Advertisements

Similar presentations

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Advertisements

Effects of early-life exposure to allergens and bacteria on recurrent wheeze and atopy in urban children Susan V. Lynch, PhD, Robert A. Wood, MD, Homer.

Vitamin D over the first decade and susceptibility to childhood allergy and asthma Elysia M. Hollams, PhD, Shu Mei Teo, PhD, Merci Kusel, MBBS, PhD, Barbara.

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Association of physical activity, asthma, and allergies: A cohort of farming and nonfarming children Bettina Bringolf-Isler, MD, PhD, Eva Graf, MD, Marco.

Latent class analysis reveals clinically relevant atopy phenotypes in 2 birth cohorts Alexander J. Hose, MA, MPH, Martin Depner, PhD, Sabina Illi, PhD,

Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications Elena Goleva,

Eosinophilic airway inflammation in asthmatic patients is associated with an altered airway microbiome Asger Sverrild, MD, PhD, Pia Kiilerich, MSc, PhD,

Inflammatory phenotypes in patients with severe asthma are associated with distinct airway microbiology Steven L. Taylor, BSc, Lex E.X. Leong, PhD, Jocelyn.

Effects of early-life exposure to allergens and bacteria on recurrent wheeze and atopy in urban children Susan V. Lynch, PhD, Robert A. Wood, MD, Homer.

Prenatal and early-life exposures alter expression of innate immunity genes: The PASTURE cohort study Georg Loss, MSc, Sondhja Bitter, MD, Johanna Wohlgensinger,

Georg Loss, PhD, Martin Depner, PhD, Laurien H. Ulfman, PhD, R. J

Increased regulatory T-cell numbers are associated with farm milk exposure and lower atopic sensitization and asthma in childhood Anna Lluis, PhD, Martin.

Markus J. Ege, MD, David P. Strachan, MD, William O. C. M

Atopic sensitization in the first year of life

Antti E. Seppo, PhD, Chloe A. Autran, MSc, Lars Bode, PhD, Kirsi M

Effect of probiotics in prevention of atopic dermatitis is dependent on the intrinsic microbiota at early infancy Ekaterina Avershina, PhD, Raul Cabrera.

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Joe K. Gerald, MD, PhD, Roni Grad, MD, William C. Bailey, MD, Lynn B

Prediction of the incidence, recurrence, and persistence of atopic dermatitis in adolescence: A prospective cohort study Astrid S. Peters, MD, MSc, Jessica.

Vitamin D over the first decade and susceptibility to childhood allergy and asthma Elysia M. Hollams, PhD, Shu Mei Teo, PhD, Merci Kusel, MBBS, PhD, Barbara.

Vijay R. Ramakrishnan, MD, Leah J. Hauser, MD, Leah M

Rising prevalence of asthma is sex-specific in a US farming population

Functional FCGR2B gene variants influence intravenous immunoglobulin response in patients with Kawasaki disease Sadeep Shrestha, PhD, Howard W. Wiener,

Majda Dzidic, MSc, Thomas R

Dynamics of the nasal microbiota in infancy: A prospective cohort study Moana Mika, MSc, Ines Mack, MD, Insa Korten, MD, Weihong Qi, PhD, Suzanne Aebi,

Test for Respiratory and Asthma Control in Kids (TRACK): A caregiver-completed questionnaire for preschool-aged children Kevin R. Murphy, MD, Robert.

The National Biome Initiative: An allergy perspective

Lower airway microbiota and mycobiota in children with severe asthma

Physician needs in health informatics: Just ask the docs

Furry pets modulate gut microbiota composition in infants at risk for allergic disease Merja Nermes, MD, PhD, Akihito Endo, PhD, Jasmin Aarnio, BM, Seppo.

Gut microbiome variations during hematopoietic stem cell transplant in severe combined immunodeficiency Jonathan P. Lane, MBBS, Christopher J. Stewart,

Early-life gut microbiome composition and milk allergy resolution

Benjamin A. Turturice, BS, Diane R. Gold, MD, MPH, Augusto A

Azithromycin therapy during respiratory syncytial virus bronchiolitis: Upper airway microbiome alterations and subsequent recurrent wheeze Yanjiao Zhou,

Jon Genuneit, MD, MSc Journal of Allergy and Clinical Immunology

Debra J. Palmer, PhD, Thomas R. Sullivan, BMa&CompSc(Hons), Michael S

Low diversity of the gut microbiota in infants with atopic eczema

Staphylococcus aureus colonization is associated with wheeze and asthma among US children and young adults Meghan F. Davis, PhD, Roger D. Peng, PhD,

Altered gene expression profiles in nasal respiratory epithelium reflect stable versus acute childhood asthma Jesus R. Guajardo, MD, MHPE, Kathleen W.

Is eczema really on the increase worldwide?

Sheena D. Brown, PhD, MSCR, Katherine M. Baxter, BS, Susan T

Kirsten M. Kloepfer, MD, MS, Vishal K

Karen C. Dannemiller, PhD, Janneane F. Gent, PhD, Brian P

A cluster-randomized trial shows telephone peer coaching for parents reduces children's asthma morbidity Jane M. Garbutt, MB, ChB, Yan Yan, MD, PhD,

H. William Kelly, PharmD Journal of Allergy and Clinical Immunology

Autophagy: Nobel Prize 2016 and allergy and asthma research

Opposite effects of CD14/-260 on serum IgE levels in children raised in different environments Waltraud Eder, MD, Walt Klimecki, PhD, Lizhi Yu, MD, Erika.

News Beyond Our Pages Journal of Allergy and Clinical Immunology

Geographic variability in childhood asthma prevalence in Chicago

A single intervention for cockroach control reduces cockroach exposure and asthma morbidity in children Felicia A. Rabito, PhD, MPH, John C. Carlson,

Prenatal exposure to a farm environment modifies atopic sensitization at birth Markus Johannes Ege, MD, Ileana Herzum, MD, Gisela Büchele, MPH, Susanne.

Baochen Shi, PhD, Nathanael J

Association between climate factors, pollen counts, and childhood hay fever prevalence in the United States Jonathan I. Silverberg, MD, PhD, MPH, Marc.

Development of atopic dermatitis according to age of onset and association with early- life exposures Caroline Roduit, MD, MPH, Remo Frei, PhD, Georg.

Toll-like receptor 2 as a major gene for asthma in children of European farmers Waltraud Eder, MD, Walt Klimecki, PhD, Lizhi Yu, MD, Erika von Mutius,

Filaggrin mutations, atopic eczema, hay fever, and asthma in children

Usa Tantibhaedhyangkul, MD, Carla M. Davis, MD, Lenora M

Protection from childhood asthma and allergy in Alpine farm environments—the GABRIEL Advanced Studies Sabina Illi, PhD, Martin Depner, PhD, Jon Genuneit,

Association of physical activity, asthma, and allergies: A cohort of farming and nonfarming children Bettina Bringolf-Isler, MD, PhD, Eva Graf, MD, Marco.

Genetic variants in the GATA3 gene are not associated with asthma and atopic diseases in German children Kathrin Suttner, MSc, Martin Depner, MSc, Norman.

The protective effect of farm milk consumption on childhood asthma and atopy: The GABRIELA study Georg Loss, MSc, Silvia Apprich, PhD, Marco Waser, PhD,

Edith Chen, PhD, Madeleine U. Shalowitz, MD, Rachel E

Increased food diversity in the first year of life is inversely associated with allergic diseases Caroline Roduit, MD, MPH, Remo Frei, PhD, Martin Depner,

Pradeep Reddy Marri, PhD, Debra A. Stern, MS, Anne L

Farming environments and childhood atopy, wheeze, lung function, and exhaled nitric oxide Oliver Fuchs, MD, Jon Genuneit, MD, Philipp Latzin, MD, PhD,

Advances in pediatric asthma in 2007

Negative affect, medication adherence, and asthma control in children

Natural history of cow’s milk allergy

Toll-like receptor heterodimer variants protect from childhood asthma

Presentation transcript:

Bacterial microbiota of the upper respiratory tract and childhood asthma Martin Depner, PhD, Markus J. Ege, MD, Michael J. Cox, PhD, Sarah Dwyer, PhD, Alan W. Walker, PhD, Lena T. Birzele, Jon Genuneit, MD, Elisabeth Horak, MD, Charlotte Braun-Fahrländer, MD, Hanna Danielewicz, MD, PhD, Raina M. Maier, PhD, Miriam F. Moffatt, PhD, William O. Cookson, MD, Dick Heederik, PhD, Erika von Mutius, MD, Antje Legatzki, PhD Journal of Allergy and Clinical Immunology Volume 139, Issue 3, Pages 826-834.e13 (March 2017) DOI: 10.1016/j.jaci.2016.05.050 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Sampling sites and timing. A, Anatomic location of the throat (T) and nasal (N) sampling site. B, Distribution of sampling time points of the nasal and throat samples. Methodical differences in sample processing preclude direct comparison of throat and nasal samples. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Multiple rarefaction curves for richness (A) and Shannon index (B) for all 327 throat (T) and 68 nasal (N) samples, respectively. Shannon index is shown up to a rarefaction of only 9100 sequences per sample. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Taxonomic composition of the microbiota from throat and nose. Survey-weighted relative abundance of phyla (A) and genera (B) for throat (T) and nasal (N) samples. Genera belonging to the same phylum are represented by the color of the respective phylum. Phyla and genera representing less than 0.5% of all reads for phyla and less than 1% for genera in the combined throat and nasal sample, respectively, are grouped in “rare.” Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Bacterial diversity in throat (T) and nasal (N) samples from children with asthma and controls. Shown are β-diversity as unweighted UniFrac (A), and α-diversity as richness (B) and Shannon index (C) for throat samples and nasal samples. The P values for Wilcoxon test comparing asthma versus control group are shown. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Relative abundance of Moraxella OTU 1462 in children with asthma and controls stratified for farming. The P value refers to the association with asthma in the respective stratum. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Inverse correlation of richness and relative abundance of Moraxella OTU 1462 in nasal samples stratified for farming. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Study design. Shown are the final throat (N = 327) and nasal (N = 68) study populations and their derivations. The recruitment population consisting of children with asthma and control children from the Bavarian GABRIELA study population was the origin of the 2 analysis populations. For 4 children of the recruitment population, no asthma information was available (*). Stratified random sampling was used. N is the number of children for the different groups in the respective population. Note that samples with low read counts after sequencing and filtering (<1000 reads) were removed in the final study population. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Number of sequences per sample for the 3 analysis populations after denoising and filtering. At the right border, sum, mean, and SD of sequences are reported. Methodical differences in sample processing preclude direct comparison of throat and nasal samples. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Taxonomic composition of the throat microbiota based on 327 and 68 children, respectively. The lower panel is restricted to the throat samples of the 68 children who also provided nasal samples. Shown is the survey-weighted relative abundance of phyla (A) and genera (B). Genera belonging to the same phylum are represented by the color of the respective phylum. Phyla and genera representing less than 0.5% of all reads for phyla and less than 1% for genera in the combined throat and nasal sample, respectively, are grouped in “rare.” Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Taxonomic composition and richness stratified for age, sampling month, and clinical conditions. Shown is the relative abundance of genera (A) and the boxplots for richness (B) in throat (T) and nasal (N) samples, respectively. The P values of Wilcoxon or Kruskal-Wallis tests refer to comparisons across strata. All values refer to unweighted analyses. Methodical differences in sample processing preclude direct comparison of throat and nasal samples. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E5 Number of bacterial 16S rRNA gene copies in throat and nasal samples. The analyses are based on 327 throat and 68 nasal samples, grouped by asthma occurrence and farm exposure. The P values of survey-weighted Wilcoxon tests refer to comparisons between asthma cases and controls. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E6 Bacterial diversity in throat (T) and nasal (N) samples from children with asthma and controls based on samples with adequate reads. Shown are β-diversity as unweighted UniFrac (A), and α-diversity as richness (B) and Shannon index (C) for throat samples and nasal samples. Adequate read counts were defined by the median of reads, that is, 4635 reads for throat samples and 8120 reads for nasal samples. The P values for Wilcoxon tests comparing asthma versus control group are shown. Journal of Allergy and Clinical Immunology 2017 139, 826-834.e13DOI: (10.1016/j.jaci.2016.05.050) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions