Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed Julien Bouley, PhD, Rachel Groeme, MSc, Maxime Le Mignon,

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Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed Julien Bouley, PhD, Rachel Groeme, MSc, Maxime Le Mignon, PhD, Karine Jain, MSc, Henri Chabre, PhD, Véronique Bordas-Le Floch, PhD, Marie-Noëlle Couret, PhD, Laetitia Bussières, MSc, Aurélie Lautrette, PhD, Marie Naveau, PhD, Véronique Baron-Bodo, PhD, Vincent Lombardi, PhD, Laurent Mascarell, PhD, Thierry Batard, PhD, Emmanuel Nony, PhD, Philippe Moingeon, PhD Journal of Allergy and Clinical Immunology Volume 136, Issue 4, Pages 1055-1064 (October 2015) DOI: 10.1016/j.jaci.2015.03.001 Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Detection of IgE-reactive proteins in short ragweed pollen extract. A-D, 2D gels stained with fluorescent dye (Fig 1, A) or probed with individual patient's serum IgE (Fig 1, B-D). Numbers refer to IgE-reactive spots (see Table E1). pI, Isoelectric point. E, One-dimensional gel probed with anti–Amb a 11 pAb (lane 1) or serum IgE (lane 2). M, MW markers. F, Percentage of allergic donors with IgE reactivity to Amb a 1 and Amb a 11 in Western blot analyses. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Primary structure of Amb a 11 and alignment with homologous allergens. A, Pre/pro-sequence. Green, red, and blue dashes indicate N-terminal signal peptide and N-terminal and C-terminal propeptides, respectively. Black circles, Amino acid variants V/I205 G/A/V357 and D/E360; black triangles, first and last residues of the mature form; magenta stars, N-glycosylation site (N127); red stars, often highly conserved residues in the protease inhibitor I29 domain. B, Alignment of the mature form with homologous allergens (see the Methods section). N-glycosylation site (magenta stars; N19), active site (green stars; C47, H181, and N202), peptidase C1A domain (orange circles; Q41, G187, W204, G209, and G212), and cysteine (triangles/open circles) residues are highlighted. Dots represent gaps. Residues colored in red or yellow are identical or partially conserved. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Purification of natural pollen allergen Amb a 11. Left panel, The mature form was purified by means of off-gel electrophoresis (OGE) and macroporous reversed-phase HPLC. Right panel, One-dimensional gel (F13 fraction, lanes 1, and ragweed pollen extract, lanes 2) probed with anti–Amb a 11 pAb or serum IgE from a patient sensitized to Amb a 11 (* and **) and Amb a 8 (arrow). M, MW markers; mRPC, macroporous reversed-phase chromatography; pI, isoelectric point. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Analysis of Amb a 11 by using MS. A, Fragmentation of peptide D14FIYANVTK22 carrying the N-glycan MMXF3 at position N19. Sugar oxonium ions and glycopeptides fragments are indicated. Blue squares, N-acetylglucosamine; green circles, mannose; red triangles, fucose; and yellow stars, xylose. B, NanoLC-MS analysis of Amb a 11. The observed molecular masses are reported in Fig 5. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Isoforms and glycoforms of Amb a 11. Calculated (§) and experimental (§§) average molecular masses (considering 7 oxidized cysteine residues) were determined by using nanoLC-MS. The residue numbering corresponds to the mature protein. The oxidized form is +15.99 Da. Blue squares, N-acetylglucosamine; green circles, mannose; red triangles, fucose; yellow stars, xylose. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 IgE reactivity with nAmb a 11 and rAmb a 11 molecules. A, **37-kDa mature nAmb a 11 (lane 1) and nonglycosylated rAmb a 11 (°°45-kDa proform, lane 2) resolved on a 1-dimensional gel (Coomassie stained [CBB]) and probed with anti–Amb a 11 pAb or serum IgE 086 (negative control) reacting only to nAmb a 1 (arrow). B, Amb a 11 IgE reactivity with sera from 4 representative donors (see also Fig E3). Lanes 1, Ragweed pollen; lanes 2, rAmb a 11. M, MW markers. C, Dot blot analysis of patients' IgE reactivity (n = 67) with 100 ng of either purified nAmb a 11, rAmb a 11, or nDer p 1 (negative control). n + or n − and r + or r −, Positive or negative reactivity to nAmb a 11 and rAmb a 11, respectively. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 7 Functional characterization of Amb a 11. A, Allergen-induced basophil activation (percentage of CD203cbrightCRTh2+ basophils) in blood samples from 5 patients. Negative (−) and positive (+) controls were used. B, Proforms (lane 1) and mature rAmb a 11 (lane 2) were resolved on a one-dimensional gel, and protease activity was monitored with (+) or without (−) cysteine protease inhibitor. M, MW markers. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 8 Model of Amb a 11 3-dimensional structure. A and B, Ribbon of Amb a 11 (model; Fig 8, A) and Der p 1 (PDB-ID 3RVW; Fig 8, B). Active site, peptidase C1A domain, and cysteine residues, as well as the termini and secondary structure elements of Amb a 11, are labeled. Residues are numbered according to the mature protein. C, Three-dimensional superposition of Amb a 11 and Der p 1. Journal of Allergy and Clinical Immunology 2015 136, 1055-1064DOI: (10.1016/j.jaci.2015.03.001) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions