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Lupine allergy: Not simply cross-reactivity with peanut or soy

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Presentation on theme: "Lupine allergy: Not simply cross-reactivity with peanut or soy"— Presentation transcript:

1 Lupine allergy: Not simply cross-reactivity with peanut or soy
Kim A.B.M. Peeters, MD, Julie A. Nordlee, MS, André H. Penninks, PhD, Lingyun Chen, PhD, Richard E. Goodman, PhD, Carla A.F.M. Bruijnzeel-Koomen, MD, PhD, Sue L. Hefle, PhD, Steve L. Taylor, PhD, André C. Knulst, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 120, Issue 3, Pages (September 2007) DOI: /j.jaci Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 India ink–stained polyvinylidene difluoride blot. Lanes 1-4, Extracts (50 μg of protein) of green pea, lupine, peanut, and soybean, respectively; lane 5, molecular weight standards. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Autoradiograms of IgE immunoblots for patients 1 to 3 with allergies to both peanut and lupine. Lanes 1-4, Extracts of green pea, lupine, peanut, and soybean, respectively; kD, molecular weight standards. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Autoradiograms of IgE immunoblots for patients with allergy to lupine but not to peanut. Lanes 1-4, Extracts of green pea, lupine, peanut, and soybean, respectively; kD, molecular weight standards. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Autoradiograms of IgE immunoblot inhibition. Upper panels: lanes 1-3, extracts of peanut, lupine, and birch pollen, respectively, used as inhibitors. Patient sera and treatments: 1N, serum 1 with no inhibitor; 1L, serum 1 with lupine extract; 3N, serum 3 with no inhibitor; 3P, serum 3 with roasted peanut extract; 3L, serum 3 with lupine extract. Lower panels: lanes 1-4, extracts of green pea, lupine, birch pollen, and soybean, respectively, used as inhibitors. Patient sera and treatments: 5N, serum 5 with no inhibitor; 5G, serum 5 with green pea extract; 5L, serum 5 with lupine extract; 5B, serum 5 with birch pollen extract; 5S, serum 5 with soybean extract. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Two-dimensional gel protein stain and IgE binding. A, Coomassie blue–stained gel of lupine extract separated by means of isoelectric focusing (pI values estimated), followed by SDS-PAGE of separated proteins in the second dimension. B, Polyvinylidene difluoride membrane incubated with patient serum 5 and indicating IgE-binding proteins. Numbers correspond to Table E1 (see this article's Online Repository at Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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