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Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

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Presentation on theme: "Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,"— Presentation transcript:

1 Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds 
Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD, Francisca Gómez, MD, Natalia Blanca, MD, PhD, Gabriela Canto, MD, PhD, Miguel Blanca, MD, PhD, Rosalía Rodríguez, PhD, Mayte Villalba, PhD, Oscar Palomares, PhD  Journal of Allergy and Clinical Immunology  Volume 133, Issue 6, Pages e4 (June 2014) DOI: /j.jaci Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A, SDS-PAGE for total kiwi, kiwi pulp, and kiwi seed extracts and Coomassie blue staining (CBS) or IgE reactivity of sera from patients allergic to kiwi (Group 1, patients with positive SPT responses to kiwifruit extract; Group 2, patients with negative SPT responses to kiwifruit extract). B, MS profile of the indicated proteins. Inset, SDS-PAGE of kiwi seed extract and CBS to visualize the excised bands (indicated arrows). Identified peptides are shown. The shadow arrows indicate peptides identified by means of MS/MS analysis. C, IgE reactivity of sera from patients allergic to kiwifruit of group 1 and 2 (lanes 1-55) to kiwi seed extract. C1 and C2 are sera from a nonatopic subject and a patient with pollen allergy, respectively. Act d 12 and Act d 13 are indicated by arrows. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 A, SDS-PAGE and Coomassie blue staining of kiwi seed extract, purified Act d 12, and purified Act d 13. B, Induced wheal size in patients with kiwifruit allergy (A) and nonallergic control subjects (NA) by SPTs with kiwi seed extract, purified Act d 12, purified Act d 13, and histamine (positive control). The horizontal discontinuous line shows the cutoff for the negative (PBS) control. Each symbol represents a single patient or healthy subject. C, Representative example of the forearm of a patient with kiwifruit allergy with positive SPT responses to the indicated allergens. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 A, Amino acid sequence alignment of 11S globulin from Actinidia chinensis (UNIPROT accession no. A0EM47) and the peptides of Act d 12 determined by means of Edman degradation and MS/MS. B, Amino acid sequence alignment of Sin a 1, 2S albumin from yellow mustard seeds (UNIPROT accession no. P15322), and the peptides of Act d 13 determined by using Edman degradation, MS, and MS/MS. Identical amino acids are shown in gray. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 IgE-binding profile to homemade total kiwifruit extract of the 55 patients with kiwifruit allergy from group 1 (lanes 1-28) and group 2 (lanes 29-55). C1 and C2 are sera from a nonatopic subject and a patient with pollen allergy, respectively. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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