Presentation is loading. Please wait.

Presentation is loading. Please wait.

Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity  Eva Maria Schimek,

Published byFanny Tedja Modified over 4 years ago

Similar presentations

Presentation on theme: "Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity  Eva Maria Schimek,"— Presentation transcript:

1 Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity  Eva Maria Schimek, MD, Bettina Zwölfer, Peter Briza, PhD, Beatrice Jahn-Schmid, PhD, Lothar Vogel, PhD, Stefan Vieths, PhD, Christof Ebner, MD, Barbara Bohle, PhD  Journal of Allergy and Clinical Immunology  Volume 116, Issue 6, Pages (December 2005) DOI: /j.jaci Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Pepsin and trypsin digestion profiles of PR-10–like allergens. M, Marker; lane 0, untreated allergen; lane E, both enzymes alone; lanes 1-3, allergen plus pepsin for 1, 5, and 30 seconds; lanes 4-7, allergen plus trypsin for 1, 5, 15, and 30 minutes; lanes 8-11, allergen plus trypsin for 30, 45, 60, and 120 minutes. The upper panels show the Coomassie staining of a 20% gel. The lower panels depict the IgE binding of a serum pool of 10 individuals with birch pollen allergy, as determined by means of immunoblotting. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Mediator release induced by digested allergens. RBL cells were loaded with serum IgE from 3 different individuals with OAS to apples, hazelnuts, and celery. The percentage of mediator release (x-axis) induced by equal concentrations of intact allergens (filled circles) and allergens after gastrointestinal digestion (open circles) is shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Proliferative responses to digested allergens. PBMCs of allergic (upper panel) and nonallergic (lower panel) individuals were stimulated with entire allergens and allergens after gastrointestinal digestion. Dpm, Delta cpm. Background levels ranged from 893 to 6566 cpm in allergic individuals and from 1215 to 8086 cpm in healthy individuals. ∗P < .05, Mann-Whitney U test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 Proteolytic fragments detected after gastrointestinal digestion of allergens. Peptides of greater than 7 amino acid residues are highlighted in gray in the sequence of each allergen. Amino acids identical with Bet v 1 are indicated in bold, and homologous residues are indicated in italics. The frames highlight known T-cell epitopes of Bet v 1 matching proteolytic fragments. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Download ppt "Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity  Eva Maria Schimek,"

Similar presentations

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.
Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,
Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS 

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS 
Biologic allergen assay for in vivo test allergens with an in vitro model of the murine type I reaction  Andreas Hoffmann, VMD, Stefan Vieths, PhD, Dieter.

Biologic allergen assay for in vivo test allergens with an in vitro model of the murine type I reaction  Andreas Hoffmann, VMD, Stefan Vieths, PhD, Dieter.
Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,

Linear epitope mapping of peanut allergens demonstrates individualized and persistent antibody-binding patterns  Christian S. Hansen, PhD, Martin Dufva,
Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens  Kirsten Beyer,

Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens  Kirsten Beyer,
Luise Westernberg, PhD, Véronique Schulten, PhD, Jason A

Luise Westernberg, PhD, Véronique Schulten, PhD, Jason A
High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE  Elizabeth A. Erwin,
Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.
Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E

Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E
Tolerant beekeepers display venom-specific functional IgG4 antibodies in the absence of specific IgE  Eva-Maria Varga, MD, Fahima Kausar, MSc, Werner.

Tolerant beekeepers display venom-specific functional IgG4 antibodies in the absence of specific IgE  Eva-Maria Varga, MD, Fahima Kausar, MSc, Werner.
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Sarcoplasmic calcium-binding protein is an EF-hand–type protein identified as a new shrimp allergen  Rosalía Ayuso, MD, PhD, Galina Grishina, MS, María.

Sarcoplasmic calcium-binding protein is an EF-hand–type protein identified as a new shrimp allergen  Rosalía Ayuso, MD, PhD, Galina Grishina, MS, María.
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Plantago lanceolata: An important trigger of summer pollinosis with limited IgE cross- reactivity  Gabriele Gadermaier, PhD, Stephanie Eichhorn, MSc, Eva.

Plantago lanceolata: An important trigger of summer pollinosis with limited IgE cross- reactivity  Gabriele Gadermaier, PhD, Stephanie Eichhorn, MSc, Eva.
Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,
Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy  Julie Wang, MD,

Similar presentations

Ads by Google