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Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy  Véronique Schulten, PhD,

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Presentation on theme: "Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy  Véronique Schulten, PhD,"— Presentation transcript:

1 Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy  Véronique Schulten, PhD, Victoria Tripple, BSc, John Sidney, MSc, Jason Greenbaum, PhD, April Frazier, PhD, Rafeul Alam, MD, David Broide, MD, Bjoern Peters, PhD, Alessandro Sette, PhD  Journal of Allergy and Clinical Immunology  Volume 134, Issue 5, Pages (November 2014) DOI: /j.jaci Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Cytokine production by PBMCs in response to the TG extract and peptide pools. Peptides that induce the production of IL-5 (A and B), IFN-γ (C and D), and IL-10 (E and F) by PBMCs from 20 patients receiving SIT and 20 controls. Panels A, C, and E show the percentage of PBMC samples from patients who responded to each antigen. Panels B, D, and F show average responses of PBMCs from 20 patients to each antigen. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Cytokine production by PBMCs from control subjects and patients who received SIT in response to TG antigens. Circles indicate the number of SFCs in PBMCs from individual donors (black circles, from 20 controls; white circles, from 20 patients who received SIT), indicating the production of IL-5 (A), IFN-γ (B), and IL-10 (C) in response to the TG extract, known allergens, and additional TG antigens. *P < .05, **P < .005, based on the Student t test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 IL-5 production by PBMCs in response to modulated ATGAs. The left panel shows the number of responding patients who received SIT (white bar) and controls (black bar) whose PBMCs produced IL-5 in response to a subset of ATGAs that show modulated IL-5 production after SIT treatment. The right panel shows the magnitude of the IL-5 response to the subset of antigens in PBMCs from patients who received SIT (white bar) or control (black bar) subjects. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Two-dimensional (2D) gel electrophoresis and immunoblot analysis of the TG extract, using pooled serum samples from controls and patients who received SIT. Coomassie-stained 2D gel of the TG extract (A). Immunoblot analysis shows proteins that react with IgG (green spots), IgE (red spots), or both (yellow spots) in sera from controls (B) and patients who received SIT (C). Yellow circles indicate locations of the 13 ATGAs that induce IL-5 production in PBMCs from controls. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Modulation of cytokine production by PBMCs from patients who received SIT in response to the MNA pool. A, Cytokine production (measured as SFCs) from patients receiving SIT (white dots) or controls (black dots). Line indicates median values. *P < .05, ***P < .001, determined by Student t test. B, IL-5 production by PBMCs from controls (black dots, left), patients who did not respond to SIT or were uncertain of their response (white dots, middle), or patients who reported improved symptoms after receiving SIT (dots with X, right) in response to the MNA pool. The dashed line (at 100 SFCs) indicates a strong response. C, A bar chart showing the percentage of patients in each group who had PBMC responses of more than 100 SFCs for IL-5. *P < .05, determined by Fisher exact test. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E1 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E2 Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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