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Identification of wheat gliadins as an allergen family related to baker's asthma  Cordula Bittner, MD, Britta Grassau, MS, Karsten Frenzel, PhD, Xaver.

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Presentation on theme: "Identification of wheat gliadins as an allergen family related to baker's asthma  Cordula Bittner, MD, Britta Grassau, MS, Karsten Frenzel, PhD, Xaver."— Presentation transcript:

1 Identification of wheat gliadins as an allergen family related to baker's asthma 
Cordula Bittner, MD, Britta Grassau, MS, Karsten Frenzel, PhD, Xaver Baur, MD  Journal of Allergy and Clinical Immunology  Volume 121, Issue 3, Pages (March 2008) DOI: /j.jaci Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Selection of the enriched clones after 3 rounds of affinity selection. After preparation of plasmid DNA, a restriction analysis was performed with EcoRI and XhoI. A selection of the inserts, ranging between 500 and 800 bp, is shown. The gliadin insert is indicated with an arrow (lane 1). The residue of pJuFo is indicated with an asterisk. Lane M, Size standard; lanes 1 through 7, pJuFo clones after EcoRI/XhoI digestion. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Nucleotide and deduced amino acid sequence of the gliadin clone. A, The nucleotide sequence analyses of 435 bp as a part of the clone PI-63 is shown. B, sequence alignment of αβ-gliadin (accession no. X02540) and our clone (PI-63) is demonstrated. Residues shared by both of them are shaded in black. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 SDS-PAGE analysis of the recombinant αβ-gliadin. The heat-denatured proteins were separated in an SDS gel by means of electrophoresis and stained either with Coomassie Brilliant Blue (A) or InVision His-tag In-gel Stain (B). The enriched recombinant αβ-gliadin is indicated with an arrow. Lane M, Molecular mass marker; lane 1, induced E coli culture; lane 2, purified eluate; lane 3, noninduced E coli culture as control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 IgE reactivity to wheat flour/total gliadin of the CAP-positive sera. The IgE reactivity to wheat flour and gliadin was determined by means of ImmunoCAP. One hundred ten of 153 sera from patients with baker's asthma revealed antibodies to wheat flour, and 50 showed antibodies to total gliadin. Two (4%) of the 50 sera positive to gliadin were negative to wheat flour (false-negative result). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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