TGF-β1 induces ILK activity in renal tubular epithelial cells.

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TGF-β1 induces ILK activity in renal tubular epithelial cells. TGF-β1 induces ILK activity in renal tubular epithelial cells. (A and B) In vitro kinase assay shows that TGF-β1 induced a biphasic activation of ILK kinase activity. HKC-8 cells were incubated with TGF-β1 at the concentration of 2 ng/ml for various periods of time as indicated. Total cell lysates were immunoprecipitated with polyclonal anti-ILK antibody. Kinase assay was performed using myelin basic protein (MBP) as the substrate. (A) Representative assay. (B) Graphic presentation of the kinetics of ILK activation. (C and D) TGF-β1 also induced ILK expression in a delayed manner. Total cell lysates were immunoblotted with specific antibodies against ILK and actin, respectively. (C) Representative Western blot. (D) Graphic presentation of relative ILK protein levels (fold induction versus controls). Yingjian Li et al. JASN 2009;20:1907-1918 ©2009 by American Society of Nephrology