1. Inhibition of ILK by QLT-0267 suppresses type I and type III collagen expression and ameliorates interstitial collagen deposition and renal fibrosis after UUO. (A) Representative RT-PCR analysis of renal mRNA levels of type I and type III collagen in differ...
Inhibition of ILK by QLT-0267 suppresses type I and type III collagen expression and ameliorates interstitial collagen deposition and renal fibrosis after UUO. (A) Representative RT-PCR analysis of renal mRNA levels of type I and type III collagen in different groups as indicated. Numbers denote each individual mouse in a given group. (B) Quantitative determination of renal mRNA levels of interstitial collagens by real-time RT-PCR in various groups as indicated. Relative mRNA levels (fold induction versus sham controls) were presented. *P < 0.05 versus sham controls; †P < 0.05 versus vehicle controls. (C through E) Representative micrographs show the deposition of collagen as detected by Picrosirius red staining. (C) Sham controls. (D) UUO 7d. (E) UUO 7d injected with QLT Bar = 40 μm. (F) Inhibition of ILK by QLT-0267 reduced tissue total collagen deposition in the obstructed kidney at 7 d after UUO. Total collagen was measured and expressed as the ratio of collagen per total protein (μg/mg). *P < 0.05 versus sham controls; †P < 0.05 versus vehicle.
Yingjian Li et al. JASN 2009;20:
©2009 by American Society of Nephrology