1. NM-3 induces p21 levels and down-regulation of Cdk2 activity.
NM-3 induces p21 levels and down-regulation of Cdk2 activity. MCF-7, ZR-75-1, and PA-1 cells were treated with the indicated concentrations of NM-3 for 48 h. A, total cell lysates were analyzed by immunoblotting (IB) with anti-p21 and anti-β-actin. B, cell lysates were subjected to immunoprecipitation with anti-Cdk2. The immunoprecipitates were incubated with histone H1 and [γ-32P]ATP. The reaction products were analyzed by SDS-PAGE and autoradiography.
Li Yin et al. Mol Cancer Ther 2001;1:43-48
©2001 by American Association for Cancer Research