1. PINCH-1 forms a complex with integrin-linked kinase (ILK) at focal adhesion sites.
PINCH-1 forms a complex with integrin-linked kinase (ILK) at focal adhesion sites. (A) Co-immunoprecipitation demonstrated a ternary complex formation among PINCH-1, ILK, and α-parvin. HKC-8 cell lysates were immunoprecipitated with specific antibody against α-parvin, followed by immunoblotting with antibodies against PINCH-1, ILK, and α-parvin, respectively. mIgG, control mouse IgG. (B) Immunofluorescence staining exhibited the co-localization of PINCH-1 and ILK at the focal adhesion sites. HKC-8 cells were transiently transfected with green fluorescence protein (GFP)-tagged PINCH-1 plasmid, followed by staining with anti-ILK and anti-paxillin antibodies. Arrows indicate the co-localization sites at focal adhesions. Bar = 5 μm.
Yingjian Li et al. JASN 2007;18:
©2007 by American Society of Nephrology