Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection

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Presentation transcript:

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection Sally Clark, MSc, Emma Page, MB BS, Tom Ford, PhD, Rebecca Metcalf, PhD, Anton Pozniak, MD, Mark Nelson, FRCP, Donald C. Henderson, PhD, David Asboe, FRCP, Frances Gotch, PhD, Brian G. Gazzard, MD, Peter Kelleher, PhD Journal of Allergy and Clinical Immunology Volume 128, Issue 4, Pages 838-846.e5 (October 2011) DOI: 10.1016/j.jaci.2011.05.025 Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, CD3+CD4+ lymphocytes were identified. B, CCR4−CXCR3+ and CCR4+CXCR3− CD4 T-cell subsets were identified. C, CCR4−CXCR3+ and CCR4+CXCR3− subsets were further subdivided into TH1/TH17, TH1, TH17, and TH2 subsets based on their CCR6 expression as follows: CD4+CCR4−CXCR3+CCR6+ (TH1/TH17 cells), CD4+CCR4−CXCR3+CCR6− (TH1 cells), CD4+CCR4+CXCR3−CCR6+ (TH17 cells), and CD4+CCR4+CXCR3−CCR6− (TH2 cells). D, Expression of CCR5 on TH1/TH17, TH1, TH17, and TH2 subsets. The frequency of positive events within each gating region is shown. FSC, Forward scatter; SSC, side scatter. Journal of Allergy and Clinical Immunology 2011 128, 838-846.e5DOI: (10.1016/j.jaci.2011.05.025) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 The proportion (A) and mean fluorescent intensity (MFI; B) of CCR5 expression on TH1/TH17, TH1, TH17, and TH2 cells from healthy control subjects (HC) and ART+ and ART− patients is shown. The horizontal line denotes the median value for each subset. Statistically significant differences between each subset were determined by using the Wilcoxon signed-rank test, and P values were reported. Analysis of the data includes all outlier points; however, exclusion of outlier points did not alter the significance of the results. Journal of Allergy and Clinical Immunology 2011 128, 838-846.e5DOI: (10.1016/j.jaci.2011.05.025) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 The frequency (top panel) and number (bottom panel) of TH1/TH17 CCR5+, TH1 CCR5+, TH17 CCR5+, and TH2 CCR5+ CD4 T-cell subsets in healthy control subjects (HC) and ART+ and ART− patients. The horizontal line denotes the median value for each subset. Statistically significant differences in CCR5+ cells of each subset between each group were determined by using the Mann-Whitney U test, and P values were reported. Analysis of the data includes all outlier points; however, exclusion of outlier points did not alter the significance of the results. Journal of Allergy and Clinical Immunology 2011 128, 838-846.e5DOI: (10.1016/j.jaci.2011.05.025) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 The relationship between the magnitude of IFN-γ and IL-2 PPD responses and the number of TH1/TH17 and TH1/TH17 CCR5+ cells in healthy control subjects and HIV-1–infected patients. The best-fit line for the relationship is shown. The Spearman rho correlation coefficient, partial correlation coefficient (when controlling for CD4 count), and significant P values are reported. SFC, Spot-forming cells. Journal of Allergy and Clinical Immunology 2011 128, 838-846.e5DOI: (10.1016/j.jaci.2011.05.025) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Unstimulated cells and SEB stimulated controls serve as the negative and positive controls for intracellular cytokine detection. Within each quadrant the percentage of IFN-γ+, IL-2+ and IFN-γ+/IL-2+ cells are indicated. The number of CD4+ or CD8+ T cells viewed within each dot plot is indicated on the top, right of each dot plot. The gating strategy incorporated a time gate to check for integrity of sample acquisition, followed by a singlet gate to exclude doublets. Lymphocytes were selected on the basis of forward and side scatter properties and T cell were selected on the basis of CD3 expression. CD4 and CD8 T cells were then identified on the CD3 gate. Gates that for the discrimination of positive and negative cytokine events were set on unstimulated PBMC stained for cytokine. The gate was placed half a log above the negative population to control for nonspecific binding of cytokine by dead, dying cells and an overestimation of true antigen specific cytokine responses. Journal of Allergy and Clinical Immunology 2011 128, 838-846.e5DOI: (10.1016/j.jaci.2011.05.025) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions