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Probiotic supplementation has little effect on peripheral blood regulatory T cells  Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary.

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Presentation on theme: "Probiotic supplementation has little effect on peripheral blood regulatory T cells  Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary."— Presentation transcript:

1 Probiotic supplementation has little effect on peripheral blood regulatory T cells 
Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary S. Warren, PhD, Suzanne Asad, BSc (Hons), Amanda J. Cox, PhD, Sampo J. Lahtinen, PhD, Markus J. Lehtinen, PhD, Peter A. Fricker, MD, Allan W. Cripps, PhD, Barbara Fazekas de St Groth, PhD  Journal of Allergy and Clinical Immunology  Volume 138, Issue 6, Pages e7 (December 2016) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Analysis of Treg-cell frequency in the peripheral blood of participants receiving Bl-04, NCFM and Bi-07, or a placebo supplement for 150 days. A and B, Percentage change in the frequency of CD127loFoxP3+ Treg cells (Fig 1, A) and CD127loCD25+ Treg cells (Fig 1, B) as a percentage of CD4+ T cells. There were nonsignificant approximately 1% to 7% differences between the groups. C-F, Frequency of circulating Treg-cell subsets in all study subjects at days 0 and 150. C, CD127loFoxp3+ Treg cells. D, CD127loCD25+ Treg cells. E, Effector/memory CXCR3+ Treg cells. F, Naive CXCR3+ Treg cells. There was a significant approximate 12% to 15% increase in Treg cells and a significant approximate 2% to 5% decrease in effector/memory and naive CXCR3+ Treg cells. G and H, Percentage change in the frequency of circulating Treg cells between participants at low, moderate, and high exercise loads, calculated as the product of the average duration of exercise by the average intensity of exercise over the course of the study. The increase in (Fig 1, G) CD127loFoxp3+ Treg cells was significantly greater in the moderate group (18%) compared with the low- (4.5%) and high-exercise groups (11%). A similar pattern is evident for (Fig 1, H) CD127loCD25+ Treg cells, but only the difference between the low- (6%) and moderate- (20%) intensity groups reached statistical significance. Whiskers represent the minimum and maximum values. Journal of Allergy and Clinical Immunology  , e7DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig E1 PBMCs were isolated from human blood and examined for the expression of markers that allowed for the identification of Treg cells (CD4, CD25, CD127 and FoxP3) and then the further examination of subsets (CXCR3+ and CCR9+). A FSC-A versus time gate was used to exclude artefacts due to fluidics instability at the start and end of the samples, and doublets were excluded using sequential FSC-A versus FSC-H and SSC-A versus SSC-H gates. Dead cells staining for Near IR were excluded before gating (gates not shown). CD4+ cells were then gated (A) and Treg cells identified as CD127loCD25+ (B) or CD127loFoxp3+ (C). CD4+ T cells and Treg cells were then subdivided as effector/memory (CD45RO+) or naive (CD45RO−). Expression of CXCR3+ was determined using a quad gate (D-G). CCR9 was not expressed (H-K). Journal of Allergy and Clinical Immunology  , e7DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E2 PBMCs were isolated from human blood and examined for the expression of markers as described in Fig E1. Calculated Treg cell percentages, based on gating for CD4+CD25+Foxp3+ and CD4+CD127loCD25+ are plotted for each sample. The Pearson correlation between the 2 measures was with a P value < Journal of Allergy and Clinical Immunology  , e7DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E3 Percentage change in the frequency of effector/memory and naive Treg cells and the frequency of CXCR3-expressing effector/memory and naive Treg cells from participants receiving Bl-04, NCFM & Bi-07 or a placebo supplement for 150 d. All Treg cells were gated as CD127loCD25+. Effector/memory Treg cells (A) and naive Treg cells (B) gated on CD45RO versus CD4, effector/memory CXCR3+ expressing Treg cells (C) and naive CXCR3+ Treg cells (D) gated on CD45RO and CXCR3. No significant differences were observed between the groups. Whiskers represent minimum and maximum values. Journal of Allergy and Clinical Immunology  , e7DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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