Presentation is loading. Please wait.

Presentation is loading. Please wait.

Human group 2 innate lymphoid cells do not express the IL-5 receptor

Published byUtami Sumadi Modified over 5 years ago

Similar presentations

Presentation on theme: "Human group 2 innate lymphoid cells do not express the IL-5 receptor"— Presentation transcript:

1 Human group 2 innate lymphoid cells do not express the IL-5 receptor
Adam K.A. Wright, PhD, Cathryn Weston, PhD, Batika M.J. Rana, PhD, Christopher E. Brightling, PhD, FRCP, David J. Cousins, PhD  Journal of Allergy and Clinical Immunology  Volume 140, Issue 5, Pages e4 (November 2017) DOI: /j.jaci Copyright © 2017 The Authors Terms and Conditions

2 Fig 1 CD125 protein is absent on blood ILC2s. A, Total PBMCs, singlets within boxed region. B, Singlet light scatter with lymphocyte gate overlaid. C, Lineage−, CD294+ cells were identified (boxed region) encompassing (D) CD123+ basophils and CD123− ILC2s, in the upper and lower left quadrant, respectively, with CD125 GMFI values. E, ILC2 light scatter properties. F, Overlay of basophil and ILC2 CD125-stained cells in the absence (red) or presence (blue) of rhIL-5. (G) Basophil and (H) ILC2 CD125 GMFI paired data (mean ± SD) from 6 healthy volunteers (open) and 13 (filled) volunteers with asthma, also shown in (I) unpaired on the same axes. Statistical comparisons were made using a Wilcoxon matched-pairs signed rank test. APC, Allophycocyanin; FITC, fluorescein isothiocyanate; FSC-A, forward scatter-area; FSC-H, forward scatter-height; SSC-A, side scatter-area. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

3 Fig 2 CD125 protein is absent on lung-derived ILC2s. A, Total lung tissue cells, singlets within boxed region. B, Singlet light scatter properties with lymphocyte gate overlaid. C, Viable CD45+ cells (boxed region). D, Lineage− CD294+ cells (boxed region). E, ILC2s (CD123−CD127+) and basophils (CD123+CD127−) were identified. (F) Basophil CD125 fluorescence and (G) ILC2 CD125 fluorescence in the absence (red) or presence of rhIL-5 (blue). H, Collated basophil and ILC2 CD125 GMFI (mean ± SD) data (n = 7). I, Proportion of CD125+ basophils and ILC2s (calculated using SED); exemplar values given in Fig 2, F and G. Statistical comparisons were made using a Wilcoxon matched-pairs signed rank test. FITC, Fluorescein isothiocyanate; FSC-A, forward scatter-area; FSC-H, forward scatter-height; SSC-A, side scatter-area. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

4 Fig E1 ILC2s do not express IL-5Rα transcripts. A, Schematic representation of IL-5Rα mRNA showing the splice variants and probe sets Hs _m1 (detecting soluble and transmembrane variants) and Hs _m1 (transmembrane only) used in this study. ΔCT values were calculated relative to 18S mRNA for (B) all IL-5Rα transcript variants using the Hs _m1 probe and (C) IL-5 transcripts using Hs _m1. In Fig E1, B and C, data are derived from isolated ILC2s (n = 3) that were grown for up to 14 days in the presence of rhIL-2, rhIL-7, rhIL-25, and rhIL-33. In Fig E1, B, cDNA generated from freshly isolated eosinophils was also included as a positive control in each IL-5Rα assay. In Fig E1, C, IL-5 transcript data are shown to confirm activation in the presence of cytokines; low ΔCT values reflect high abundance of the IL-5 transcript. n.d., Not detected. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

Download ppt "Human group 2 innate lymphoid cells do not express the IL-5 receptor"

Similar presentations

Increased numbers of activated group 2 innate lymphoid cells in the airways of patients with severe asthma and persistent airway eosinophilia  Steven.

Increased numbers of activated group 2 innate lymphoid cells in the airways of patients with severe asthma and persistent airway eosinophilia  Steven.
Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity  Laetitia Everaere, PhD, Saliha Ait-Yahia, PhD, Olivier Molendi-Coste,

Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity  Laetitia Everaere, PhD, Saliha Ait-Yahia, PhD, Olivier Molendi-Coste,
CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,

CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,
Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.
Tan Jinquan, MD, PhD, Henrik H. Jacobi, MD, Chen Jing, MD, Claus M

Tan Jinquan, MD, PhD, Henrik H. Jacobi, MD, Chen Jing, MD, Claus M
Vitamin D enhances production of soluble ST2, inhibiting the action of IL-33  Paul E. Pfeffer, MRCP, Yin-Huai Chen, MSc, Grzegorz Woszczek, PhD, Nick C.

Vitamin D enhances production of soluble ST2, inhibiting the action of IL-33  Paul E. Pfeffer, MRCP, Yin-Huai Chen, MSc, Grzegorz Woszczek, PhD, Nick C.
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
Immunologic defects in 22q11.2 deletion syndrome

Immunologic defects in 22q11.2 deletion syndrome
The inhibitory effects of topically active glucocorticoids on IL-4, IL-5, and interferon-γ production by cultured primary CD4+ T cells  Shelby P. Umland,

The inhibitory effects of topically active glucocorticoids on IL-4, IL-5, and interferon-γ production by cultured primary CD4+ T cells  Shelby P. Umland,
Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,
Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

Ramses Ilarraza, PhD, Yingqi Wu, Christopher D
Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,

Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,
FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria.

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria.
Type 2 innate lymphoid cells in induced sputum from children with severe asthma  Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.

Type 2 innate lymphoid cells in induced sputum from children with severe asthma  Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.
IL-33 induces innate lymphoid cell–mediated airway inflammation by activating mammalian target of rapamycin  Robert J. Salmond, PhD, Ananda S. Mirchandani,

IL-33 induces innate lymphoid cell–mediated airway inflammation by activating mammalian target of rapamycin  Robert J. Salmond, PhD, Ananda S. Mirchandani,
Neonatal rhinovirus induces mucous metaplasia and airways hyperresponsiveness through IL-25 and type 2 innate lymphoid cells  Jun Young Hong, MS, J. Kelley.

Neonatal rhinovirus induces mucous metaplasia and airways hyperresponsiveness through IL-25 and type 2 innate lymphoid cells  Jun Young Hong, MS, J. Kelley.
Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells  Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.
Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge  Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge  Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,
Flow cytometric measurement of STAT1 and STAT3 phosphorylation in CD4+ and CD8+ T cells—clinical applications in primary immunodeficiency diagnostics 

Flow cytometric measurement of STAT1 and STAT3 phosphorylation in CD4+ and CD8+ T cells—clinical applications in primary immunodeficiency diagnostics 
Cysteinyl leukotriene E4 activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D2 and epithelial cytokines  Maryam Salimi,

Cysteinyl leukotriene E4 activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D2 and epithelial cytokines  Maryam Salimi,

Similar presentations

Ads by Google