Presentation is loading. Please wait.

Presentation is loading. Please wait.

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria.

Published byRaoul Malo Modified over 5 years ago

Similar presentations

Presentation on theme: "FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria."— Presentation transcript:

1 FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria A. Johansson, PhD, Lena Hell, MSc, Gintare Lasaviciute, MSc, Caroline Nilsson, MD, PhD, Ulrika Holmlund, PhD, Eva Sverremark-Ekström, PhD  Journal of Allergy and Clinical Immunology  Volume 138, Issue 3, Pages e4 (September 2016) DOI: /j.jaci Copyright © 2016 The Authors Terms and Conditions

2 Fig 1 The phenotype and functional responses of CD4+CD25+FOXP3+CD127low cells mature with age. PBMC were analyzed after 2-hour resting (A and D) or after 24-hour stimulation with S aureus-CFS (B and E). Naive CD4+ T cells were cultured with autologous monocytes and analyzed after 48-hour stimulation with S aureus-CFS (C). Fig 1, A, Left: the percentage of CD25+FOXP3+CD127low (FOXP3+) cells among the CD4+ T-cell population. Middle and right: the percentages of CD45RA+ cells (middle) and HELIOS+ cells (right) among the FOXP3+ population. Fig 1, B, Upper left: the percentage of FOXP3+ cells among the CD4+ T-cell population; upper right and lower panels: the percentages of IL-10+, IFN-γ+, and IL-17A+ cells among the FOXP3+ population. Fig 1, C, Representative intracellular stainings of FOXP3 and T-bet expression in isolated naive CD4+ T cells or in CD4+ T cells in whole PBMCs. Fig 1, D, The percentage of CD161+ cells among the FOXP3+ population. Fig 1, E, The percentage of CD161+ cells and CD161 MFI values among the FOXP3+ population. CB, Cord blood; MFI, mean fluorescence intensity; T-bet, T-box transcription factor. ∗P < .05, ∗∗P ≤ .01, ∗∗∗P ≤ .001, and ∗∗∗∗P ≤ Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

3 Fig 2 Infant colonization with S aureus and lactobacilli associates with CD161 expression and cytokine production by FOXP3+ cells at 2 years of age. PBMC were analyzed after 2-hour resting (A and B) or after 24-hour stimulation with S aureus-CFS ± L reuteri-CFS (C-E). Immunological data at 2 years of age were correlated with the presence/absence of gut bacteria early in life (age 1 week, 2 weeks, 2 months). Fig 2, A and B, The percentage of CD161+ cells among the FOXP3+ population in 2-year-old children colonized (+) or not colonized (−) by S aureus or in correlation with the relative amounts of S aureus in stool expressed as percent bacterial DNA of total nucleic acids. Fig 2, C, The percentage of IL-10+ cells among the FOXP3+ population in correlation with the relative amounts of S aureus in stool. Fig 2, D, The percentage of FOXP3+ cells expressing IL-10, IFN-γ, and IL-17A. Fig 2, E, The percentage of IL-10+ cells among the FOXP3+ population in 2-year-old children in relation to lactobacilli colonization. ∗P < .05, ∗∗P ≤ .01, ∗∗∗P ≤ .001, and ∗∗∗∗P ≤ Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

4 Fig E1 Representative dot plots of flow cytometry data. A, PBMCs were rested for 2 hours before being stained and analyzed by flow cytometry. Left: staining of FOXP3 and CD25 gated from live CD4+ T cells; middle and right: staining of HELIOS (middle) and CD45RA (right) gated on CD4+CD25+FOXP3+CD127low cells. B, PBMCs were left unstimulated or cultured in the presence of S aureus 161:2-CFS. Representative staining of FOXP3 and CD25 exemplified by PBMCs from one 7-year-old donor. C, PBMCs were left unstimulated or cultured in the presence of S aureus-CFS. Representative intracellular staining of IL-10 expression in FOXP3+ cells. D, Representative intracellular stainings of ROR-γt and GATA-3 expression in isolated naive CD4 T cells cultured with autologous monocytes or in CD4 T cells in whole PBMCs after stimulation with S aureus 161:2-CFS. CB, Cord blood; FSC, forward scatter; GATA, trans-acting T-cell-specific transcription factor GATA-3; ROR, RAR-related orphan receptor. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

5 Fig E2 The percentage of CD4+ and CD4+FOXP3− T-cells that express CD45RA, HELIOS, and CD161 alter with age. PBMC were rested for 2 hours before being stained and analyzed by flow cytometry. A-C, The percentage of CD45RA+ cells (Fig E2, A), HELIOS+ cells (Fig E2, B), and CD161+ cells (Fig E2, C) among the total CD4+ (left column) or the CD4+FOXP3− (right column) T-cell populations. CB, Cord blood. ∗P < .05, ∗∗P ≤ .01, ∗∗∗P ≤ .001, and ∗∗∗∗P ≤ Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

6 Fig E3 L reuteri-CFS dampen S aureus-CFS–induced cytokine production. PBMCs were left unstimulated or cultured in the presence of S aureus-CFS ± L reuteri-CFS. A, The percentage of CD161+ cells among the FOXP3+ population. B, Representative intracellular staining of IL-10 expression in FOXP3+ cells. C, Secreted levels of IL-10, IFN-γ, and IL-17A in PBMC cultures measured with ELISA. Secreted levels of all cytokines at unstimulated conditions were with no exceptions below the detection limits (not shown). FSC, Forward scatter. ∗P < .05, ∗∗P ≤ .01, ∗∗∗P ≤ .001, and ∗∗∗∗P ≤ Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 The Authors Terms and Conditions

Download ppt "FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization  Sophia Björkander, Maria."

Similar presentations

Monica G. Lawrence, MD, Jennifer W. Leiding, MD, Jonathan J

Monica G. Lawrence, MD, Jennifer W. Leiding, MD, Jonathan J
Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 
Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,

Vitamin D3 treatment of vitamin D–insufficient asthmatic patients does not alter immune cell function  Brandy Reid, MS, Pierre-Olivier Girodet, MD, PhD,
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 
Human group 2 innate lymphoid cells do not express the IL-5 receptor

Human group 2 innate lymphoid cells do not express the IL-5 receptor
Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu  Amanda J. Reefer, MS, Kathryn.
Regulatory T cells as a biomarker for response to adalimumab in rheumatoid arthritis  Dao X. Nguyen, BSc, Alice Cotton, RN, BSc, Laura Attipoe, MBBS, Coziana.

Regulatory T cells as a biomarker for response to adalimumab in rheumatoid arthritis  Dao X. Nguyen, BSc, Alice Cotton, RN, BSc, Laura Attipoe, MBBS, Coziana.
Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response  Miriam Wittmann, MD, Mareike Alter, Tanja.
Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 
Sytze de Roock, PhD, Arie J

Sytze de Roock, PhD, Arie J
Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,
Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis  Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis  Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.
Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of TH1/TFH signatures  Jelle de Wit, PhD, Tineke Jorritsma,

Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of TH1/TFH signatures  Jelle de Wit, PhD, Tineke Jorritsma,
Immunologic defects in 22q11.2 deletion syndrome

Immunologic defects in 22q11.2 deletion syndrome
Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,
T-cell regulation during viral and nonviral asthma exacerbations

T-cell regulation during viral and nonviral asthma exacerbations
Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal- dominant gain-of-phosphorylation STAT1 mutations  Pamela P.W. Lee,

Penicillium marneffei infection and impaired IFN-γ immunity in humans with autosomal- dominant gain-of-phosphorylation STAT1 mutations  Pamela P.W. Lee,

Similar presentations

Ads by Google