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Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses  Stephan Traidl, Petra Kienlin,

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Presentation on theme: "Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses  Stephan Traidl, Petra Kienlin,"— Presentation transcript:

1 Patients with atopic dermatitis and history of eczema herpeticum elicit herpes simplex virus–specific type 2 immune responses  Stephan Traidl, Petra Kienlin, Gabriele Begemann, Lichen Jing, PhD, David M. Koelle, MD, Thomas Werfel, MD, Lennart M. Roesner, PhD  Journal of Allergy and Clinical Immunology  Volume 141, Issue 3, Pages e5 (March 2018) DOI: /j.jaci Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Cytokine production of TCLs and polarization analysis of virus-specific TH cells. A, TCLs from patients with AD compared with healthy individuals (n = 16) were grown in the presence of antigens as indicated on the x-axis. Cytokines were detected by ELISA in cell culture supernatants. Only those TCLs are depicted that showed increased antigen-specific proliferation in restimulation testing. B, Patients with AD shown in Fig 1, A, were differentiated into ADEH− and ADEH+. C and D, Stimulated CD4+ cells were detected by CD154 magnetic bead enrichment and characterized for CXCR3, CCR4, CCR6, and CCR10. C, Patients with AD compared with healthy individuals. D, Patients with AD were differentiated into ADEH− and ADEH+. Flu, Influenza. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Polarization of virus-specific TC cells and intracellular IL-4 and IFN-γ staining of HSV1 UL25 MHC class I tetramer+ cells. A and B, Stimulated CD8+ cells were detected by CD137 phycoerythrin (PE) magnetic bead enrichment and characterized for CXCR3, CCR4, and CCR6. A, Patients with AD compared with healthy individuals. B, Patients with AD shown in Fig 2, A, were differentiated into ADEH− and ADEH+. C, Intracellular cytokine staining of healthy, ADEH−, and ADEH+ donors for IFN-γ and IL-4 in HSV1 UL25 MHC class I tetramer+ cells. Flu, Influenza; ns, not stimulated. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 T-cell surface marker expression on virus-specific T cells. A, Gating strategy of the surface marker analysis specifically of antigen-activated CD154+ CD4+ cells for CXCR3, CCR4, CCR6, and CCR10. B, Increased CD4+CD154+ cell frequencies after 7- to 24-hour stimulation. Stimulation as indicated; exemplary plots are shown. C, Gating strategy of the surface marker analysis specifically of antigen-activated CD137+ CD8+ cells for CXCR3, CCR4, and CCR6. D, Increased CD8+CD137+ cell frequencies after 24- to 28-hour stimulation. Stimulation as indicated; exemplary plots are shown. FSC-A, Forward scatter-area; Flu, influenza; FSC-W, forward scatter-width; SSC-A, side scatter-area. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 HSV1 UL25 MHC class I tetramer+ cells were analyzed for intracellular IL-4 and IFN-γ. A, Increase in tetramer+ cells after 14-day stimulation with the UL25 peptide compared with nonstimulated T cells at day 14. B, Intracellular cytokine staining of healthy, ADEH−, and ADEH+ donors for IFN-γ and IL-4. C, Direct ex vivo tetramer staining in combination with intracellular cytokine staining. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 Patient's characteristics. Age in years and total IgE in kU/L for healthy, ADEH−, and ADEH+ patients. SCORing Atopic Dermatitis values were measured for patients with AD. Absence of significant differences between ADEH− and ADEH+ patients in the displayed characteristics. NA, Nonatopic. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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