Supplemental Table 3. Oligonucleotides for qPCR Sequence – 5’ to 3’ Tm ˚C Genome Position HV68 M1 Forward Outer ATG CAG CTG GCC ACC TTA TG 58.1°C γHV68: 2023-2042 HV68 M1 Reverse Inner TCC GC TTC TCG AAG GAA TCA G 56.5°C γHV68: 2228-2207 HV68 M2 Forward Inner TAA GGA CCT CGT AGA GAT TGG C 55.8°C γHV68: 4193-4214 HV68 M2 Reverse Inner ACG TTA AAG TCC CCA TGG AAG C 57.5°C γHV68: 4336-4315 HV68 M3 Seq Forward TAC TCC TCC ACC TTT ACC TG 53.1°C γHV68: 6626-6645 HV68 M3 Seq Reverse CTG TTC TTA CAG ACC GGG G 55°C γHV68: 6545-6563 18s Forward AGA TCA AAA CCA ACC CGG TGA 56.8°C N/A 18s Reverse GGT AAG AGC ATC GAG GGG GC 60.1°C