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Supplemental Table S1 For Site Directed Mutagenesis and cloning of constructs P9GF:5’ GAC GCT ACT TCA CTA TAG ATA GGA AGT TCA TTT C 3’ P9GR:5’ GAA ATG.

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Presentation on theme: "Supplemental Table S1 For Site Directed Mutagenesis and cloning of constructs P9GF:5’ GAC GCT ACT TCA CTA TAG ATA GGA AGT TCA TTT C 3’ P9GR:5’ GAA ATG."— Presentation transcript:

1 Supplemental Table S1 For Site Directed Mutagenesis and cloning of constructs P9GF:5’ GAC GCT ACT TCA CTA TAG ATA GGA AGT TCA TTT C 3’ P9GR:5’ GAA ATG AAC TTC CTA TCT ATA GTG AAG TAG CGT C 3’ LAP9GF:5’ CCC GGA TCC ATC ACT ATA GAT AGG AAG TTC ATT TCA TTT G 3’ LAP9GR:5’ CAA ATG AAA TGA ACT TCC TAT CTA TAG TGA TGG ATC CGG G 3’ PRP9GF:5’ TGG ATC CTC ACT ATA GAT AGG AAG TTC ATT TCA TTT GG 3’ PRP9GR:5’ CCA AAT GAA ATG AAC TTC CTA TCT ATA GTG AGG ATC CA 3’ AtrbcS9GF:5’ CAT TGT TGT TAT CAT TAT AGA TAG ATG ACC AAA GCA CTA GAC C 3’ AtrbcS9GR:5’ GGT CTA GTG CTT TGG TCA TCT ATC TAT AAT GAT AAC AAC AAT G 3’ For sequencing of constructs T3:5’ AAT TAA CCC TCA CTA AAG GG 3’ T7:5’ TAA TAC GAC TCA CTA TAG GG 3’ P-10:5’ ACA GCA ATT GCC CGG CTT TCT TGT AA 3 For detection of nucleosome TCPF1: 5’ TGT TGT GTG GAA TTG TGA GCG 3’ TCPR1G: 5’ TGA ACT TCC TAT CTA TAG TGA GG 3’ TCPF2:5’ GAA TTG TGA GCG GAT AAC AAT TT 3’ TCPR2G:5’ TAT CTA TAG TGA GGA TCC TCT AG 3’ TCPF1:5’ TGT TGT GTG GAA TTG TGA GCG 3’ TCPR3:5’ AAT GAA ATG AAC TTC CTA TAT ATA GT 3’ TCPF4:5’ TTT CAC ACA GGA AAC AGC TAT G 3’ TCPR4:5’ GTT GAA AAA TGA CAA CAC GTG TC 3’ NTP303F 5′ TCC TCA GAG GGA TTT CCC TT 3′ NTP303R 5′ CAA GAA GAA GAG GAG ACG CTT 3′ For Semiquantitative RT-PCR uidAF:5’ GCA CTG CCA TGG TCC GTC CTG TAG AAA CC 3’ uidAR:5’ CGA TCC CGG GTC ATT GTT TGC CCC CTG CTG C 3’

2 Contd …Supplemental Table S1 For quantitation of promoters in Arabidopsis A. Reference Gene (Ubiquitin) At4G05320F: 5’ CAC ACT CCA CTT GGT CTT GCG T 3’ At4G05320R: 5’ TGG TCT TTC CGG TGA GAG TCT TCA 3’ B. Primer pairs of the 9 genes in Arabidopsis At3G03626F: 5’ TGA GTC ACA AAT GTA ATT TCC ATA AAA GAG 3’ At3G03626R: 5’ ACA AAT CCG CCA CGT TAT GC 3’ At1G15960F: 5’ TCT GCT ACG TAA CGT TGT GGG TAA A 3’ At1G15960R: 5’ AAG AAA CCC CTG CAT AAC GTA TTG 3’ At3G46830F: 5’ TCA GGT TGA AGG AAA GAC CAT TAA GG 3’ At3G46830R: 5’ TCG TAG ACA AGA AGT GCA CCC A 3’ At4G27870F: 5’ ATG TAG ACA AGG AAA CAG AGA ACC AAA A 3’ At4G27870R: 5’ CTG ATT GAG CAT TAG GTG GCA AC 3’ At1G62935F: 5’ AAA ACC GTG ATG ATG ATG ATA ATC ATG 3’ At1G62935R: 5’ TTT GTC GGT TCC TCT CTC TTC TC 3’ At1G32510F: 5’ ACT TCA AGG GAG ACT TCG CGC 3’ At1G32510R: 5’ CTT TTC TAG GTC ACT TGC TTG ACA 3’ At1G27490F: 5’ GGG GAA ACC AAG TGG TTT CTT G 3’ At1G27490R: 5’ ACG TTG ATC CAC TTG TAC GTG TTT ATT 3’ At1G22800F: 5’ TTG GTG GAG AAA CCT TAA AGG AGC 3’ At1G22800R: 5’ TCA ACA GAT TCC CTG CGT CC 3’ At4G19720F: 5’ CCT TCC ATT AAA GGT CCA TGC G 3’ At4G19720R: 5’ GAG AGT CCA GGA CCA GCC AAC 3’

3 Supplemental Table S5 * For the TATA consensus motif, NFM was used from Molina and Grotewold (2005) # For all other motifs NFMs were made so that there is no degeneracy in the sequence 50 bp Upstream Sequences in Arabidopsis Total Number of Protein Coding Genes by TAIR database Total Number of Genes by TAIR database 27379%33281% TATA(T/A)A(T/A)A Consensus * NFM Derived from MEME 321811.75350810.54 * NFM Derived from PlantProm 477817.45529415.91 TATATATA # Nucleotide Frequency Matrix (NFM) 9903.6210773.24 TATAGATA # Nucleotide Frequency Matrix (NFM) 480.18570.17 TATACATA # Nucleotide Frequency Matrix (NFM) 1270.461450.44 TATAAATA # Nucleotide Frequency Matrix (NFM) 8673.179322.80 Identification of TATA motifs in -50 bp upstream promoter sequences of protein coding and total genes in Arabidopsis thaliana


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