1. Supplemental Table 1. Oligonucleotides for plasmid construction and BAC-mediated recombination.
Sequence – 5’ to 3’
Tm ˚C
Genome Position
HV68 TMER7 Δ mt. Forward
GGC CTT TGG CCA GGG CGA GTC CTG GCC AGC
73.8°C
γHV68: – 4953 and 5019 – 5033
HV68 TMER7 Δ mt. Reverse
GCT GGC CAG GAC TCG CCC TGG CCA AAG GCC
γHV68: – 5019 and 4953 – 4939
BglII insert pEPkan-S Forward
AGA TCT GAC CAA CCC TAA GTG AGT TTT TCT TCT TGC TTA ACA CAA AAT TTT TAT CTT CGA TTT ATT CAA CAA AGC CAC G
67.9°C
N/A
BglII insert pEPkan-S Reverse
AGA TCT CGC GTA TAT CTG GCC CGT ACA TCG
63.6°C
pol II miR-M1-1 Forward
GTG TGT GCA CCC CAC TAT C
55.8°C
γHV68: 75 – 93
HV68 pol III-8 Universal Reverse
GGT CCA GGC AAA TGT GAG AAG
56.1°C
γHV68: 5,580 – 5,560
HV68 Left End Seq. #4 Forward
GCA GGC CCC ACC GCT AC
62.2°C
γHV68: 3,000 – 3,016
KanR Screen Reverse
CTT GTG CAA TGT AAC ATC AGA GAT TTT G
55.2°C
tRNA6 Reverse
GAA AAA CTC ACT TAG GGT TGG TCA GAT CTA C
58.5°C
γHV68: 3,874 – 3,844
TMER 1 HS BAC Mut Forward
TTC AAG TCC GAG CTC TGG AGG AAG TGG GTC CAA CTT TTT TCC TTT TTT GTG ATA GAA ATG GCC GTA CTC GAT TTA TTC AAC AAA GCC ACG
71.2°C
γHV68: , bold is the hard stop mutation, (underlined sequence is viral sequence)
TMER 1 HS BAC Mut Reverse
GGT TCT TAA TTA TAA AGG AAG TAC GGC CAT TTC TAT CAC AAA AAA GGA AAA AAG TTG GAC CCA CTT CCT CCG CGT ATA TCT GGC CCG TAC ATC G
70.2°C
Diebel – TMER TKO Manuscript Supplemental Table 1