Supplemental Table 1. Oligonucleotides for plasmid construction and BAC-mediated recombination. Sequence – 5’ to 3’ Tm ˚C Genome Position HV68 TMER7 Δ mt. Forward GGC CTT TGG CCA GGG CGA GTC CTG GCC AGC 73.8°C γHV68: 4939 – 4953 and 5019 – 5033 HV68 TMER7 Δ mt. Reverse GCT GGC CAG GAC TCG CCC TGG CCA AAG GCC γHV68: 5033 – 5019 and 4953 – 4939 BglII insert pEPkan-S Forward AGA TCT GAC CAA CCC TAA GTG AGT TTT TCT TCT TGC TTA ACA CAA AAT TTT TAT CTT CGA TTT ATT CAA CAA AGC CAC G 67.9°C N/A BglII insert pEPkan-S Reverse AGA TCT CGC GTA TAT CTG GCC CGT ACA TCG 63.6°C pol II miR-M1-1 Forward GTG TGT GCA CCC CAC TAT C 55.8°C γHV68: 75 – 93 HV68 pol III-8 Universal Reverse GGT CCA GGC AAA TGT GAG AAG 56.1°C γHV68: 5,580 – 5,560 HV68 Left End Seq. #4 Forward GCA GGC CCC ACC GCT AC 62.2°C γHV68: 3,000 – 3,016 KanR Screen Reverse CTT GTG CAA TGT AAC ATC AGA GAT TTT G 55.2°C tRNA6 Reverse GAA AAA CTC ACT TAG GGT TGG TCA GAT CTA C 58.5°C γHV68: 3,874 – 3,844 TMER 1 HS BAC Mut Forward TTC AAG TCC GAG CTC TGG AGG AAG TGG GTC CAA CTT TTT TCC TTT TTT GTG ATA GAA ATG GCC GTA CTC GAT TTA TTC AAC AAA GCC ACG 71.2°C γHV68: 201-220, bold is the hard stop mutation, 232-251 (underlined sequence is viral sequence) TMER 1 HS BAC Mut Reverse GGT TCT TAA TTA TAA AGG AAG TAC GGC CAT TTC TAT CAC AAA AAA GGA AAA AAG TTG GAC CCA CTT CCT CCG CGT ATA TCT GGC CCG TAC ATC G 70.2°C Diebel – TMER TKO Manuscript Supplemental Table 1