1. Supplemental Table 3. Oligonucleotides for qPCR
Sequence – 5’ to 3’
Tm ˚C
Genome Position
HV68 M1 Forward Outer
ATG CAG CTG GCC ACC TTA TG
58.1°C
γHV68:
HV68 M1 Reverse Inner
TCC GC TTC TCG AAG GAA TCA G
56.5°C
γHV68:
HV68 M2 Forward Inner
TAA GGA CCT CGT AGA GAT TGG C
55.8°C
γHV68:
HV68 M2 Reverse Inner
ACG TTA AAG TCC CCA TGG AAG C
57.5°C
γHV68:
HV68 M3 Seq Forward
TAC TCC TCC ACC TTT ACC TG
53.1°C
γHV68:
HV68 M3 Seq Reverse
CTG TTC TTA CAG ACC GGG G
55°C
γHV68:
18s Forward
AGA TCA AAA CCA ACC CGG TGA
56.8°C
N/A
18s Reverse
GGT AAG AGC ATC GAG GGG GC
60.1°C