2. AIM:

3. Genetic Engineering: changing the DNA of living organisms. 1. Inserting genes into other organisms 2. Selective Breeding 3. Cloning

4. Transgenic organism- genes from more than one species Example: GFP mice, silk goats

5. First, we must ISOLATE the gene Restriction enzymes: cut DNA at specific sequences of nucleotides EcoRI cuts at “sticky end”

6. Separating DNA Gel electrophoresis can be used to separate DNA sequences

7. Applications of Transgenic Organisms

8. Bacteria can produce insulin

9. Plasmid contains antibiotic resistance= genetic marker

10. 2. Selective Breeding

11. 3. Cloning

12. Summary: Explain the role of restriction enzymes and plasmids in genetic engineering How could genetic engineering be used to treat medical conditions?