1. Agustina Setiawati PRODUK DNA REKOMBINAN

2. Prinsip DNA rekombinan

3. STRATEGI KLONING GENA EUKARIOT  Isolasi total mRNA dari jaringan ttt  Ubah mRNA menjadi cDNA  Sisipkan pada vektor ekspresi  Transformasi  Isolasi protein produk

4. PROTEIN FARMASETIK HASIL REKOMBINAN

5. Produksi Insulin Proinsulin

9. Berg JM, et al, 2002

13. TRANSGENIC PLANT

14.  EDIBLE VACCINE  VIRUS-RESISTENT PLANT

15. EDIBLE VACCINE Edible vaccines are vaccines produced in plants that can be administered directly through the ingestion of plant materials containing the vaccine. Eating the plant would then confer immunity against diseases. Edible vaccines produced by transgenic plants are attractive for many reasons. The cost associated with the production of the vaccine is low, especially since the vaccine can be ingested directly, and vaccine production can be rapidly up scaled should the need arises. Edible vaccine is likely to reach more individuals in developing countries. The first human clinical trial took place in 1997. Vaccine against the toxin from the bacteria E.coli was produced in potato. Ingestion of this transgenic potato resulted in satisfactory vaccinations and no adverse effects.

16. What exactly are “edible vaccines?” Biopharmaceuticals Plants or crops that produce human vaccines The next generation of vaccines

17. ALUR PEMBUATAN EDDIBLE VACCINE

19. VIRUS RESISTANT PLANT

20. Transgenic Animals

21. SOMATIC NUCLEAR CELL TRANSFER  Transgenic animal was constructed based on SNCT by Robert et al (1952)

22. DOLLY SHEEP, first succeed cloning

23. Uses for transgenic animals Gene pharming Xenotransplantation Industrial Food/Feed

24.  The foreign gene is constructed using recombinant DNA technology.  In addition to a structural gene, the DNA usually includes other sequences to enable it to be incorporated into the DNA of the host, and to be expressed correctly by the cells of the host. Transgenic Animal

25. Recombinant protein production in the milk of a transgenic sheep

26. GENE THERAPY Agustina Setiawati, M.Sc., Apt

27. DEFINITION 1. Gene therapy is a technique for correcting “defective” genes responsible for disease development.

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33. Want to know more? Just ask!

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