1. Manipulating and Cloning DNA

2. Being Healthy Type 1 diabetes Type 2 diabetes How can you help these individuals?

3. Insulin Banting and Best – Pigs and cows Helen Free Allergic reaction to insulin from pigs and cows Genetic engineering – Inserted into bacteria – Produced insulin from humans

4. Why Bacteria? Reproduce quickly Plasmids Form recombinant DNA – Restriction enzymes – Sticky ends vs. blunt ends – Restriction fragments Sticky or Blunt ends?

5. How Do You Know Where to Cut? Restriction enzymes recognize recognition sites – Sticky ends & blunt ends Restriction map

6. We Are Family Vector + foreign material = recombinant DNA – Plasmids Competent/host cell takes up recombinant DNA – Bacteria Restriction enzymes cut DNA ligase reforms backbone

7. Recombinant DNA

8. Hybridization

9. Homework Page 375 # 2, 3, 5, 8