1. HPLC Instrument Manager, Europe Region 1
History of Varian HPLC
Frankie Button
HPLC Instrument Manager, Europe Region 1
November 2007

2. 1966 is where it all started……..
1966 – LC Research and Development Group of Varian was formed
LC Development and Innovation took place every 1 to 3 years.
In the early years this was mainly by product development
In later years this has been mainly by acquisition

3. History of Varian HPLC Products
1967 – Model 4000 Gas Displacement Pump and Micro-adsorption detector (MAD)

4. History of Varian HPLC Products
1968 – Varian purchased LC Division of Picker-Nuclear (Dedicated Nucleotide Analyzer, LCS-1000, 254 nm UV detector.
1971 – Model 4200 Gradient Elution Chromatograph (syringe pump), Variscan Scanning UV Detector (Techtron – Melbourne)

5. History of Varian HPLC Products
Model 8500 HPLC syringe pump, RI detector, 8000 AutoSampler for HPLC, CDS 101

6. History of Varian HPLC Products
1976 – Varichrom Variable Wavelength UV-Vis detector (Cary)
2nd Generation CDS System, with system control!

7. History of Varian HPLC Products
1977 – Fluorichrom filter fluorimeter

8. History of Varian HPLC Products
5000 series HPLC pump, major step forward
5010 Isocratic analog pump
5020 Binary gradient computer controller
5030 Isocratic computer controller
UV detector

9. History of Varian HPLC Products
Inside the 5020

10. History of Varian HPLC Products
1979 – UV-50 UV-Vis detector
1980 – vista 54/56 HPLC (Controlled by Vista 401 chromatography data station) Ternary gradient HPLC pump

11. History of Varian HPLC Products
1981 – Post Column Reactor, RI-3 (refractive index detector from LDC)
1982 – UV-100 time programmable UV-Vis detector with fast HPLC option!
1983 – 5560 HPLC, scanning UV-200 detector,
8085 integrated AutoSampler, CDS-402 for control

12. History of Varian HPLC Products
1984 – 9090 low loss variable volume AutoSampler, 2010 Isocratic pump, 2020 Gradient controller, 2050 Programmable UV-Vis detector, 2070 Monochrometer based Fluorescence detector (all from Jasco)

13. History of Varian HPLC Products
1985 – PolyChrom 9060 Diode Array Detector, AASP Automated solid phase extraction system
( Online SPE!!!)

14. History of Varian HPLC Products
1986 – CM-1 conductivity detector (OEM), DS-604 GPC calculation software
1987 – RI-4 (OEM from ERC in Japan), 2500 Isocratic pump, 2502 Isocratic pump and gradient controller, 2550 time programmable UV-Vis detector (Jasco)

15. History of Varian HPLC Products
1988 – Star 9010 ternary gradient pump, 9050 time programmable UV-Vis detector, 9095 High Performance AutoSampler, 9065 Diode Array detector, 9020 Windows based HPLC workstation

16. History of Varian HPLC Products
1989 – LC Workstation Rev C, SP-99 column oven (Spark Holland)
1990 – 3D View for PolyView, PCR carbamate system (Pickering), 9001 Isocratic pump, Marathon AutoSampler (Spark Holland)
1991 – 9070 Fluorescence Detector (Linear, Spectra Physics, Thermo), 9100 Cooled AutoSampler
1992 – MCA for PolyView
1993 – 9012/9002 extended flow range pumps, Mistral oven (Spark Holland), Electric 9100

17. History of Varian HPLC Products
1994 – Workstation version 4.0 (LC and GC combined), PolyView 4.0 (IsoView), 9300 AutoSampler (Spark Holland), Prospekt Automated solid phase extraction (Spark Holland)

18. History of Varian HPLC Products
1995 – Star 9080 Electrochemical detector ( Antec), Star 9040 RI detector (ERC), Star control of 9200 and 9300
1996 – 9025 Automation panel, LC/NMR, 9012 Quaternary pump, 9075 fluorescence detector (Thermo)
1997 – Varian buys Rainin – SD-200/300, SD-1, UV-1, UV-C, UV-D, PDA-2, AI-1A, AI-96, AI-03, RamPak, ISCO FC-1, ISCO, FC-4

19. History of Varian HPLC Products
1998 – ProStar product line ( combines Rainin and Varian products), ProStar 330 Photodiode Array (Hitachi) PolyView 2000
1999 – ProStar 500 CVM, Helix system
2000 – ProStar 363 Fluorescence detector, Varian buys Van Kel, Varian buys R&S, 520 Oven

20. History of Varian HPLC Products
2001 – PrepStar SD-2, PrepStar 530, Responder software, Helix 364 Fluorescence software, Star Reviewer for dHPLC, Polaris Educational systems
2003 – ProStar 325 UV/Vis detector

21. Key technical advances for Varian HPLC
Variscan - scanning UV-Vis detector 1971
5000 pump – single piston single pump ternary gradient system 1978
Vista 401 – 4 channel, control LC and GC, dual high speed printers, replot with baseline, raw data storage 1980
UV 100 – No RI high performance analytical product 1982
PolyChrom 9060 PDA with mathematical evaluation of purity – 1985
Star Workstation – Windows based data system, 1988
PolyView spectral processing software – 1988 (MCA 1992)
SD-1 – the Waters alliance before there was an alliance – 1991 with Rainin. (A fire truck without the wheels)
ProStar 500 CVM – The ultimate in valving capability – 1999

22. Varian products that lasted
5000 pump deign – now in ProStar 230
Vista 401 capabilities – now in Star and Galaxie
UV-100 design – Obsolete in 2003 by ProStar 325
Spectral processing software – never surpassed!

23. Products that customers loved
LC 5000 – many are still working today
Star 9010/9012/230 – same as above
Vista 401 – Incredible innovation and very easy to use
UV-100/9050/ProStar 310 – they were the workhorses of the lab
SD-1 – Unparalleled Performance
SD-200/SD-300/210/218 – the best in world for analytical to small scale prep

24. HPLC at Varian for over 40 Years
Varian have always been, and always will be an Innovator in HPLC
Even in the last 10 years we have produced product after product, and several acquisitions
Over $8M Invested in the last 4 years to put us back on the HPLC Map
This new Era starts with, because it’s the first of many NEW HPLC products:

25. The Future………..
iHPLC

26. Concept of the iHPLC 1st Fully Integrated HPLC System
Easy to use, install and MAINTAIN
Compact and Stylish in Design
Complementary to part of existing modular line
A Major step forward in performance and ergonomics and User Experience
Single part numbers, easier to sell 26 26

27. iHPLC
Two New Models
Analytical
Analytical to Prep

28. iHPLC Analytical Concept: One Power Button One Power Cable
One Communication cable
…Whilst retaining the Self maintenance features
Pre-plumbed and “Ready” out of the box
Sample tray accessible without opening doors 28 28

29. iHPLC Analytical USP: High Performance Analytical System
Can be Optimised easily for good Fast LC performance
The “First COMPLETE Integrated Solution”
Simplicity in a one Box
Simple to Install…Simple to Use....Simple to Maintain
Neat, Compact and Stylish product 29 29

30. iHPLC Analytical Consists of: NEW Pumps NEW Autosampler NEW Detectors
Existing UV/DAD Detectors in an Integrated format 30 30

31. iHPLC Analytical
USP:
The Only complete range of High Performance Information Rich LC Detectors
IMS Software for simple user diagnostics, OQ and Service
Storage space for LC Columns, Fittings and Tubing
Tray Space for Columns, manual, tools, Software CD etc… 31 31

32. iHPLC Analytical to Prep
Consists of:
Existing 210/218 Pumps in several integrated configurations
NEW Fraction Collector
NEW Prep Autosampler
NEW Module for Column and Solvent Selection
Existing UV/DAD Detectors in an Integrated format 32 32

33. iHPLC Analytical to Prep
Concept:
One Power Button/Cable
One Communication cable
Easy interchangeability of components to retain flexibility
Pre-plumbed and “Ready” out of the box 33 33

34. iHPLC Analytical to Prep
USP:
High Performance Analytical System
High Performance “Analytical to Prep” System
High Performance Prep System
The “Only COMPLETE Integrated Solution”
Accepted Hardware 34 34

35. iHPLC Analytical to Prep
USP:
Simple to Install…Simple to Use....Simple to Maintain
Neat, Compact and Stylish product
Modern, Stylish Fraction Collector…That works!
Simple FC programming and manual intervention
New Switching module concept, stylish design 35 35

36. Galaxie Driver Awesome interface
Very “Easy on the eye” –shockwave technology
Simple to use
An enormous step forward
Installation configurator – simple to install 36 36

37. Other Highlights Solvent tray capacity
“Ski Slopes” for sample tray insertion/removal
Single waste port
Easy access for servicing/tubing and fitting replacement
Leak detector 37 37

38. Other Highlights Modern Manual Injectors – push button
Bottle Management
Software Tool Tips – makes everything easy
Online Help and Videos “ Cary Style”
Excellent Brochures – hoorah a brochure!!!!!!!
New Data Sheets/Price Lists and App Notes 38 38

39. Positioning Form part of our New LC range
Still have modular options – very important
Rebranded XXX-LC, eliminate ProStar name
First product 356-LC Refractive Index plus
210/218/325/335etc..
New Grey “ Barbie Showers” 39 39

40. EXCELLENT AND COMPREHENSIVE
Positioning
Integrated Systems(iHPLC)
Standard popular configurations
New Format
Modular Systems(XXX-LC)
Low cost systems
Odd configurations
Biocompatible
Online SPE
Prep 2 Process/Flash LC
GPC Systems
Analytical
Semi-Prep
Prep
Process
GPC
Flash
Turnkey
Custom
EXCELLENT AND COMPREHENSIVE
RANGE OF CONSUMABLES 40 40

41. Conclusion Compact and Stylish Integrated Systems
Retain flexibility and maintenance features
Excellent hardware performance
Excellent User Interface – pleasant experience
Simple to Install
Fits the topical “Fast LC” Model using our Two Brand NEW Small Particle LC Columns 41 41