1. 900-LC P. van Poppel – Field Support Specialist October 2009

2. The 900 series LC Analytical 920 LCSemi-Prep 940 LC

3. What will be covered Modules/ Options Parts Replacement Strategy Installation IMS diagnostics/ Troubleshooting

4. Modules Autosampler Fluorescence Detector Quaternary Pump Column Heater/ Manual Injector Fraction Collector (external) Scale Up module MIB --------------------------------------------------------------------------------------------------- UV-vis Detector PDA Detector RI Detector (internal and external) ELSD (external) Isocratic Pump Sample Loading Pump

5. Modules Autosampler Fluorescence Detector Quaternary Pump Column Heater/ Manual Injector Scale Up module MIB And the IMS

6. Parts Replacement Strategy Ease of serviceability and a minimal number of parts will help field service representatives repair the instrument in an efficient and cost effective manner. We expect to service all modules to an assembly level, not to a sub assembly level. Component level repair of the circuit boards is not recommended. Warranty replacement of DOA modules within the system must be handled in accordance with the Instrument Escalation Procedure. Melbourne Field Support will not authorize the replacement of defective instruments unless there is an escalation.

7. Installation Hardware Network Software

8. Hardware Installation Plug & Play Pre-Plumbed Pre-Wired

9. Hardware Installation Autosampler Detector Sample vial tray screw Syringe movement lock down bracket

10. Hardware Installation Shipping Screw Fluorescence Detector (bottom view)

11. Hardware Installation

13. Network Setup

17. Software Installation

28. IMS

29. IMS Actions

30. IMS Counters

31. IMS Service Tests

32. IMS Additional Tests

33. IMS Log Book

35. IMS Help

37. Modules Autosampler Fluorescence Detector Quaternary Pump Column Heater/ Manual Injector Scale Up module MIB

38. Auto Sampler Poor Reliability Injection Valve System Injection Port System LC System Contamination Injection Valve System Injection Port System Sample LC System Electronics

39. Autosampler Electronics Electronics Not Initialising Error X, Y, Z S-Valve I-Valve System Busy/ Communications Error

40. IMS Autosampler Tests Autosampler Complete Test Autosampler Temperature Accuracy Test

41. IMS Autosampler Actions Injection port seal replaced Injection valve rotor replaced Syringe replaced Purge syringe Syringe valve rotor replaced Injection valve stator replaced Syringe valve stator replaced Needle replaced Wash needle Move to injection port Syringe needle adjustment

42. Fluorescence Detector General Issues Noise Drift Poor Signal to Noise Leak Errors Power Problems Electronics Lamp Errors Board Errors Data Errors

43. IMS Fluorescence Detector Actions 150W xenon lamp replaced Long life xenon lamp replaced Mercury lamp replaced Flowcell cleaned/replaced Wavelength Accuracy

44. IMS Fluorescence Detector Tests Signal to Noise by Raman Light Scattering Detector Noise Test

45. Quaternary Pump Problems General Issues Reproducibility Carryover No Peak Observed Mixing Ratio Accuracy Pressure Fluctuations Split Peaks Liquid does not Flow No Power Pump Motor does not Rotate

46. Quaternary Pump Problems Degasser Issues Bubbles Generated at Outlet Bubbles Generated when Pump sucks Liquid does not flow Vacuum Pump does not work Vacuum Pump does not stop

47. Quaternary Pump Electronics Electronics Motor Error Leaks Data & Board Errors Limit Errors

48. Quaternary Pump Electronics

49. IMS Quaternary Pump Actions Inlet check valve replaced Outlet check valve replaced Piston seal replaced Drain valve seal replaced Drain valve filter replaced Solenoid valve replaced Mixer filter replaced Solvent filter replaced Gradient four way joint replaced (Cam Position Adjustment)

50. IMS Quaternary Pump Tests Flow rate accuracy - Test meter method Flow rate accuracy test - Time volume method Flow Compositional accuracy test Flow Leak test Flow precision test Flow pulsation test Flow compositional Ripple Test Flow gradient linearity test Flow compositional precision test

51. Column Heater/ Manual Injector Problems General Issues Heating Problems Leaks Pressure

52. Scale Up Module Problems General Issues Leaks Pressure

53. MIB Problems General Issues Communications Power

54. IMS Column Heater/ Manual Injector Actions & Tests Injector valve stator replaced Injector valve rotor replaced Column valve stator replaced Column valve rotor replaced Column heater temperature accuracy and stability

55. IMS Scale Up Module Actions & Tests Injector valve stator replaced (Manual) Injector valve rotor replaced (Manual) Injector valve stator replaced (SUM) Injector valve rotor replaced (SUM) Column valve stator replaced (SUM) Column valve rotor replaced (SUM)

56. IMS MIB Actions & Tests Backup the system configuration to a local folder Restore the system configuration from a previous backup Full System Test

57. Autosampler Practical Person 1: X, Y, Z movement adjustment Person 2: Vial Detection & Injection Valve Sensor adjustment Person 3: X & Y movement adjustment Mechanism Rails …Injection Port Position, Syringe Valve and Syringe Sensor adjustments…

58. Fluorescence Detector Practical Person 1: Replacement of the Xenon Lamp and Positional Adjustment of the Xenon Lamp Person 2: Replacement of the Mercury Lamp Perform a linearity test Person 3: Clean the Flow Cell Perform a wavelength accuracy test

59. Pump Practical Person 1: Replacement of the Pump Seal & Drain Valve Seal and Solvent Filter Wash Person 2: Replacement & Cleaning the Inlet and Outlet check valves and Pressure Test Mode in IMS Person 3: Remove the pump and Pump Board