1. Metrohm Ion Chromatography
The new UV/Vis generation – 886/887
Dr. Katinka Ruth
Product Specialist Competence Center IC Metrohm International Headquarters
UV/Vis generation 886/887

2. The new UV/Vis generation – 886/887
Outline
New UV/Vis detector
New heated reactor
For post-column reactions
As column department
Applications
UV/Vis generation 886/887

3. 887 Professional UV/Vis Detector
Hardware
Diode array detector
UV lamp (deuterium) + Vis lamp (halogen)
Built in as in 844
8 data channels + 1 reference channel
CH1/CH2 quotient channel omitted
887 = standalone detector
Combination possible with 850, 881, 882
(≠ conductivity detector)
Connection to PC via USB
Analog out: up to 4 channels
UV detector can basically be combined with all other IC devices
UV/Vis generation 886/887

4. 887 Professional UV/Vis Detector
Features
Same functionality as 844
MagIC Net
T int, T out: New names for better understanding
Tint = integration time
Tout = measuring time
Measuring time = multiple of integration time
Records spectra at any point of the chromatogram
Graphic frame in MagIC is not able to show 3D
UV/Vis generation 886/887

5. Feature: Spectrum at any time point
Retention time 19 min (bromide)
Retention time 23 min (nitrate)
Retention time 9 min (nitrite)
2 ppm nitrite, 4 ppm bromide, 50 ppm nitrate
in artificial sea water (28 g/L NaCl)
A Supp
5 mmol/L Na2CO3, 5 mmol/l NaHCO3
1.0 mL/min
MSM MCS (step after 13.5 min)
218 ± 5 nm
Sample volume 5 µL
According to AW CH
Range 1 means 1mV = 1mAU
UV/Vis generation 886/887

6. Features Evaluate absorbance maxima Define start and end wave length
Automatic evaluation of absorbance maxima
Up to 10 automatic evaluations
Range 1 means 1mV = 1mAU
UV/Vis generation 886/887

7. Features Direct and indirect UV detection
Change polarity via MagIC Net
Range 1 means 1mV = 1mAU
UV/Vis generation 886/887

8. Features New assembly of lamps Installation from the top Easy mounting
Range 1 means 1mV = 1mAU
Deuterium lamp (UV)
Halogen lamp (Vis)
UV/Vis generation 886/887

9. 887 Professional UV/Vis detector
Technical specifications
Array with 512 diodes
Channel λ 190 – 900 nm (increment 1)
Bunching ± 0 – 100 nm (increment 1)
Integration time 10 – 254 ms
Measuring time 50 – 2000 ms
Noise (expected) 0.1 – 0.2 mAU (similar to 844)
Resolution 1.4 nm
Metal free
Leak sensor on tray
PEEK detector cell
Bunching bigger than with 844
Noise: tests with final device + software coming soon, lower noise with suppression
Optical path
UV/Vis generation 886/887

10. 887 Professional UV/Vis Detector
Possible combinations
MagIC Net 2.2 = must
Stand alone UV detector
Controlled by MagIC Net Multi, Professional, and Compact
887
Professional UV/Vis Detector
UV/Vis generation 886/887

11. 887 Professional UV/Vis Detector
Possible combinations
881 or
882
Compact IC X0
Compact IC pro X0
Compact IC plus
System Connector ProfIC/Compact IC 
Professional UV/Vis Detector
Connector
Only UV detector
Or UV detector + conductivity detector
887
Professional UV/Vis Detector
1 detector: MagIC Net Compact
2 detectors: MagIC Net Professional
UV/Vis generation 886/887

12. 887 Professional UV/Vis Detector
Possible combinations
850
Professional IC
887
Professional UV/Vis Detector
2.850.XXXX
Professional IC
Professional UV/Vis Detector σ σ
1 detector: UV
2 detectors: UV + conductivity
Needs MagIC Net Professional
3 detectors: UV + 2 x conductivity
in series or parallel detection
MagIC Net Professional
UV/Vis generation 886/887

13. New heated reactor – general info
Heating up to 150°C, increment 0.1°C
Direct heat transfer, faster heating than 850, 881
<10 min (20°C to 40°C)
Temperature stability: better than 0.05°C
Temperature reproducibility: better than ± 0.2°C
Temperature accuracy: <1°C
MagIC Net 2.2 (Professional and Multi)
Combinations possible with 850, 887, 881, 882
Host for further 872 Extension modules
Robust design
Two versions:
a) Professional Reactor
b) Professional Thermostat
Replaces PCR box
Heat-conductive paste, aluminium housing, aluminium core, spiral capillary
For column heating
UV/Vis generation 886/887

14. a) 2.886.0110 Professional Reactor
For post-column-derivatisation (PCR)
Intelligent reactor = iReactor
(with chip on reactor plate)
Temperatures up to 120°C
Space to have 4 reactors
Heated mixing-T
Accessories (included)
Y-connector 3 x UNF 10/32
Reactor plate complete to PCR Module
Reactor complete to
Metrosep BP 1 Guard/2.0
UV/Vis generation 886/887

15. a) 2.886.0110 Professional Reactor
Setup
Base plate (iReactor)
+ 1 reactor
(0.5 x 2000 mm PEEK-capillary)
dimensions as previous PCR-Box
+ 2 reactor e.g. as preheating capillary for eluent or post column reagent
Up to 4 reactors
Setup depends on application/desired temperature
Der Reaktor besteht aus einer spiralförmigen 0.5 x 2000 mm PEEK-Kapillare, welche auf einem Alu-Kern aufgewickelt ist. Die Wäme wird über eine Wärmeleitpaste vom Alu-Gehäuse auf die Kapillare übertragen. Die Dimensionen sind identisch mit der im PCR-Modul verwendeten Teflon-Mischkapillare von SeQuant. Bei der Reaktionsgeschwindigkeit und beim Rauschen wurden keine Unterschiede festgestellt.
UV/Vis generation 886/887

16. b) 2.886.0210 Professional Thermostat
Heated column department
Compatible with intelligent columns
Column length up to 150 mm
Temperature up to 80°C
Accessories (included)
Preheating capillary with core, 1.44 m
Column holder complete to PCR Module
UV/Vis generation 886/887

17. 2.886.0210 Professional Thermostat
Setup
Up to 2 columns in the thermostat
e.g. column + pre-column
or 1 x 150 mm + 1 x 100 mm
1 column is monitored
Additional pre-heating capillary must be installed
UV/Vis generation 886/887

18. Intelligent Thermostat / Reactor
Name reactor plate
Set to work
Last change
Comment
Chip for reactors as at intelligent columns
Monitoring / Traceability
1 Chip per reactor plate (iReactor)
Data field for up to 4 reactors
Reactor 1 mounted
Reactor 1 order number
Reactor 1 material
Reactor 1 length
Reactor 1 inner diameter
Reactor 1 comment
Reactor 2 mounted
Reactor 2 order number
Reactor 2 material
Reactor 2 length
Reactor 2 inner diameter
Reactor 1 comment
etc.
UV/Vis generation 886/887

19. 2.886.0110 Professional Reactor Possible combinations
881 or
882
Compact IC
Transport of PCR-reagent:
High pressure iPump of 872 Extension module
Use of BP 1 Guard X0
Compact IC pro X0
Compact IC plus
System Connector ProfIC/Compact IC 
Professional UV/Vis Detector
Professional Reactor
Extension Module IC Pump
Metrosep BP 1 Guard/2.0
Connector
887
Professional UV/Vis Detector
886
Professional
Reactor
872
IC Pump
UV/Vis generation 886/887

20. 2.886.0110 Professional Reactor Possible combinations
881 or
882
Compact IC
Transport of PCR-reagent:
881 peristaltic pump +
backpressure capillary
Regeneration MSM:
800 Dosino
Peristaltic pump of
(for non-suppressed systems)
800
Connector
aw ch Title[ Regeneration and Rinsing of the MSM suppressor with one Dosino ]
887
Professional UV/Vis Detector
886
Professional
Reactor
(2/3)0
Compact IC pro
(2/3)0
Compact IC plus
System Connector ProfIC/Compact IC 
Professional UV/Vis Detector
Professional Reactor
UV/Vis generation 886/887

21. 2.886.0110 Professional Reactor Possible combinations σ σ
Transport of PCR-reagent:
850 high pressure iPump
(alternative : peristaltic pump)
Use back pressure column
together with iPump
Flexible detector setup
850
Professional IC
887
Professional UV/Vis Detector
886
Professional Reactor σ σ
UV in series or parallel
2.850.XXXX
Professional IC
Professional UV/Vis Detector
Professional Reactor
Metrosep BP 1 Guard/2.0
UV/Vis generation 886/887

22. 2.886.0110 Professional Reactor Possible combinations
Transport of PCR-reagent:
872 Extension Module IC Pump or
872 Sample Prep (peristaltic pump)
Transport of eluent + injection:
872 Extension Module IC Module
Bottle holder + tray
887
Professional UV/Vis Detector
886
Professional
Reactor
872
IC Module
IC Pump
Professional UV/Vis Detector
Professional Reactor
Extension Module IC Module
Extension Module IC Pump
Bottle holder (ProfIC)
Tray with sensor
Metrosep BP 1 Guard/2.0
Backpressure capillary
UV/Vis generation 886/887

23. 2.886.0210 Professional Thermostat
Possible combinations
Simple stand-alone UV-system
886 as column department
Bottle holder + tray
887
Professional UV/Vis Detector
886
Professional
Thermostat
872
IC Module
Professional UV/Vis Detector
Professional Thermostat
Extension Module IC Module
Bottle holder (ProfIC)
Tray with sensor
UV/Vis generation 886/887

24. suppressed or non-suppressed
UV detection –
suppressed or non-suppressed
+MSM +MCS MSM-MCS –MSM-MCS
N = 8
500 ppb NO2, Br, NO3; A Supp (4.0 mm); 0.7 mL/min 5mM Na2CO3 0.3 mM NaOH, 45°C, 20ul loop
Noise 0.05 – mAU Noise 0.05 – mAU Noise 0.15 – 0.2 mAU
500 ppb NO2-, Br-, NO3- Mean (n = 8)
20 µL loop ± 15 nm
45°C Integration time 13 ms
A Supp 15 – 150/4.0 Measuring time 1 s
0.7 mL/min 5 mM Na2CO mM NaOH
UV/Vis generation 886/887

25. suppressed or non-suppressed
UV detection –
suppressed or non-suppressed
NO2
+MSM +MCS
+MSM
-MCS
–MSM
Full width at half maximum
0.40
0.35
Height
1.8
Area
0.79
0.80
0.70
Noise
0.09
0.18
S/N 21 10
Theoretical plates
1619
1362
2041 Br
+MSM +MCS
+MSM
-MCS
–MSM
Full width at half maximum
0.53
0.52
0.62
Height
0.7
0.5
Area
0.38
0.39
0.27
Noise
0.09
0.18
S/N 8 3
Theoretical plates
1782
1709
3068
NO3
+MSM +MCS
+MSM
-MCS
–MSM
Full width at half maximum
0.65
0.66
Height
1.5
1.1
Area
1.02
1.07
0.80
Noise
0.09
0.18
S/N 17 6
Theoretical plates
1533
1324
2335
Noise has influence on integration, and thus theoretical plates can change
Conclusion Area, height, noise, S/N = suppressed better
Theor. Plates = non-suppressed better
UV/Vis generation 886/887

26. suppressed or non-suppressed
UV detection –
suppressed or non-suppressed
Recommendation
Chemical suppression (MSM)
Improvement for
Noise → S/N
System peak
Injection peak
No MCS
Peak broadening
costs
+ MSM + MCS
+ MSM
- MCS - MSM
- MCS
UV/Vis generation 886/887

27. Applications
All existing applications published with 844 or Lambda 1010
(reactor dimensions identical to previous PCR-Box)
Direct UV/Vis detection
Indirect UV/Vis detection
Pre column derivatisation + UV/Vis detection
Post column derivatisation + UV/Vis detection
UV/Vis generation 886/887

28. Applications Examples for Post column reactions (PCR)
Transition metals
Chromate
Bromate
Silicate
Cyanide, cyanogen chloride
Aluminium
...
Potential new applications for PCR
Amino acids
Ammonium next to high amounts of sodium
Au, Ag, Hg, Pt, Pd
UV/Vis generation 886/887

29. Applications 887 Professional UV/Vis detector Cl- NO2- NO3-
500 ppb NO2-, NO3- in artificial sea water (28 g/L NaCl)
A Supp 10 – 250/4.0
5 mmol/L Na2CO3, 5 mmol/l NaHCO3
0.7 mL/min
5 µL
MSM MCS
218 nm ± 15 nm
T int 13 ms, T out 1 s
AW CH
NO2-
No dilution; Cl influence
NWG Interface, hard to judge, ~50 ppb
NO3-
UV/Vis generation 886/887

30. UV detection of nitrite and nitrate
Calibration 50 ppb – 20 ppm
Ion
Relative standard deviation
Correlation coefficient
Nitrite
1.163
Nitrate
UV/Vis generation 886/887

31. Applications Transition metals 10ppm Cu, Ni, Zn, Co, Mn,
20ppm Cd, 50ppm Pb
Metrosep C 5 – 150/4.6
15 mmol/L oxalic acid pH 3.5 (NH3)
1.0 mL/min (eluent)
0.5 mL/min (PCR)
10 µL
510 nm ± 11 nm
PCR-reagent
0.15 mmol/L PAR 0.4 mol/L NH mmol/L HNO3
More info/details presentation “columns”
NWG ~1 ppb expected
UV/Vis generation 886/887

32. Applications Amino acids USP 27 norm for amino acids Requires 120°C
Possible with new PCR reactor
0.5 mM amino acids
(20 compounds)
Metrosep Amino Acids 1 – 100/4.0
USP eluent
570 nm
PCR-reagent
Ninhydrin, hydrindatin
dimethylsulfoxid,
lithium acetate, acetic acid
(N2 purged)
UV/Vis generation 886/887

33. Applications Bromate Bromate 4.8 min According to draft norms
ISO/DIS EPA 326
PCR, tri-iodide method
UV absorbance at 352 nm
Metrosep A Supp 16 – 100/4.0
0.5 – 5 ppb Bromate
UV/Vis generation 886/887

34. Summary UV/VIS generation – 886/887 Professional UV/Vis detector
MagIC Net
8 channels (wavelength + bunching)
spectra at any time point
functionality as 844
Professional Thermostat / Reactor
Faster heating + higher temperatures (direct heat transfer)
iReactor
2 MPa backpressure for smooth adding of reagent
Available in summer 2010 (depending on MagIC Net 2.2)
Faster heating + higher temperatures compared to 850, 881, and Dionex specs
UV/Vis generation 886/887