We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you!
Presentation is loading. Please wait.
Published byMeredith Harmon
Modified over 2 years ago
Ion Chromatography HCO 3 - /CO 3 2- Eluent Eluant 0.0028M NaHCO 3 0.0022M Na CO 23 ~2 ml/min flow Pump 500 - 5,000 psi 1,000 nominal Check for air bubbles Sample Loop Sample in Syringe or from Autosampler Waste 50 ul Inject Valve Sample Guard Column Separator Column RNR 3 + HCO 3 - + Cl - RNR 3 + Cl - HCO 3 - + 2RNR 3 + CO 3 = + SO 4 = 2RNR 3 + CO 3 = + SO 4 = 4 = Cl - NO 3 - "R" = latex resin particle Conductivity Detector + - Na + HCO 3 - + - Cation Exchange Membrane H + Na + H CO 2 3 Anode Cathode - H O 2 + O 2 + 2 OH - H 2 - - Computer/Integrator Self Regenerating Anion Suppressor time Chromatogram Cl - F - SO 4 = NO 3 - water dip Conductivity (uS) PO 4 3- PO 4 3- - -
Degas Eluant Quaternary Pump 500 - 5,000 psi 1,650 psi nominal Sample Loop Sample in Syringe or from Autosampler Waste 100 l Inject Valve Sample Guard Column Separator Column RNR 3 + OH - + Cl - RNR 3 + Cl - OH - + 2RNR 3 + 2(OH - ) + SO 4 2- 2RNR 3 + + SO 4 2- Cl - NO 3 - "R" = latex resin particle Conductivity Detector + - Na + OH - + - Cation Exchange Membrane H+H+ Na + H2OH2O Anode Cathode - + + OH - - - Computer/Integrator Self Regenerating Anion Suppressor time Chromatogram Cl - F-F- SO 4 2- NO 3 - Conductivity ( S) PO 4 3- - - Ion Chromatography OH - Eluent 40 mM NaOH Degas Eluant DI Water SO 4 2- 2OH - H2OH2O H2OH2O O2O2 H2H2 PO 4 3- CO 3 2-
Eluent He Gas To Pump Pump Mode He Gas To Pump Degas Mode Eluent
Conductivity Detector + - Na + OH - + - Cation Exchange Membrane H+H+ Na + H2OH2O Anode Cathode - + + OH - - - H2OH2O H2OH2O O2O2 H2H2 - - NO 3 - H+H+ ~ pH 10 ~ pH 3
Latex Bead OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + OSO 3 - +H3N+H3N NH 3 + SO 4 2- OH - Cl - OH -
time Suppressed IC Conductivity ( S) time Non-Suppressed IC Conductivity ( S)
Capillary Ion Chromatography, Sample Analysis on Demand Salt Lake City, UT May 2012.
Ion Chromatography. Ion Exchange Separation is facilitated by formation of ionic bonds between charged samples and charged column packings.
Over view What is ion chromatography? Why this techniqu is useful?
Ion Exchange Chromatography The stationary phase has an ionically charged surface opposite to that of the analyte Factors controlling retention:
Ic_sampl_prep_ _e.ppt 1 IC Metrohm inline sample preparation Techniques for analytical success Metrohm Ion Chromatography Bart Cleeren, Metrohm.
Introduction to Ion Chromatography By Greg Langland CCSF EMSA 30A.
Laboratory technique for the Separation of mixtures Chroma -"color" and graphein - "to write”. Colour bands - separation of individual compounds.
Field Methods of Monitoring Aquatic Systems Unit 9 – Ion-Chromatography Copyright © 2006 by DBS.
Gel filtration Chromatography
PRESENTED BY:- GAIKWAD OVESH & MD.NADEEM MANSOORI
Electrolysis. What is electrolysis? Electrolysis means “breaking up a compound with electricity” From the Greek Electro – electricity Lysis – breaking.
An Improved Ion Chromatographic Method for Low Level Perchlorate Analysis Peter E. Jackson, Ph.D. Environmental Market Manager Dionex Corporation 1228.
6.5- The Strength of Acids and Bases. Strong acids A strong acid is an acid that reacts almost completely ( >99%) with water to form hydronium ions HCl.
IX.Salts and Hydrolysis Salts are simply ionic compounds. Salts can be formed by: 1.A metal reacting with a non-metal. 2 Na (s) + Cl 2(g) 2 NaCl.
Salts product of neutralization reaction 1.00 M NaOH mol OH - L mol1.00 mol L x L x = 0.075L H+H+ + Cl - + Na + + OH - H2OH2O+ Na + + Cl - NaCl.
High-Performance Liquid Chromatography HPLC, when GC won’t cut it!!!
COLUMN CHROMATOGRAPHY SMK Negeri 13 Bandung. R+OH- + X- === R+X- + OH- n R-H+ + Mn+ === (R)n-Mn+ + n H+
HSC Chemistry – Acidic Environment R Slider. * The pH of a salt depends upon the relative strength of the ions that make up the salt * Very few salts.
High Performance Liquid Chromatography. HPLC originally refered to: High Pressure Liquid Chromatography currently refers to: High Precision Liquid Chromatography.
1 Chapter 14 Acids and Bases 14.9 Acid-Base Properties of Salt Solutions Copyright © 2008 by Pearson Education, Inc. Publishing as Benjamin Cummings.
Ion-Pair Chromatography In addition to the aqueous buffer and an organic solvent that is typical for reversed-phase, the mobile phase contains a counter.
Hydrolysis and Neutralization
Standard Methods for the Examination of Water and Wastewater, 21st Ed
Chromatography Chapter 4 1 Dr Gihan Gawish. Definition Dr Gihan Gawish Ion-exchange chromatography (or ion chromatography) is a process that allows.
Perchlorate Analysis by Ion Chromatography The CA DHS Protocol H.S. Okamoto, D.K. Rishi and S.K. Perera.
Ion Exchange Chromatography
Waters HPLC Competitive Review
Chapter 33 High-Performance Liquid Chromatography.
Development of an Automated, Pyridine Free Method for Aerosol Cyanide Compounds and Hydrogen Cyanide. An Alternative to NIOSH LeRoy Dobson, Chemist.
Created with MindGenius Business 2005® Ion Exchange Chromatography Ion Exchange Chromatography Principles Small K means low affinity – so low RT K is often.
Introduction High-performance liquid chromatography (HPLC) is a form of liquid chromatography.liquid chromatography The main purpose is to separate.
Hydrogen electrolysis Hydrogen electrolysis is the process of running an electrical current through water (H 2 O) and separating the hydrogen from the.
All you need to know – in the available time!
ICS Madrid Acids, Bases and Salts Left click your mouse to continue.
Qualitative Analysis Chem 1106 Lab 19. QA Process of determining if a chemical is present or not NOT how much only the presence Changed form 5 to 3 anions.
Slide 1 of 52 Ch.16. Acids and Bases (the fundamentals)
BY: JUAN GONZALEZ MEEN 3344 DOWEX 21 K XLT Anion Exchange Resin.
HPLC – High Performance Liquid Chromatography
Dissociation and Neutralization GLE What is dissociation? The process by which an ionic compound separates into its positive ions (cations) and.
Collect 200 L groundwater from FW021 pH = 3.25 Nitrate = 1,911 mg/L Uranium = 1.38 mg/L Tc-99 = 30,974 pCi/L Peristaltic pump FW021 Plastic tank Test Solution.
Small particles (3-10 m) give high performance but require high pressure 1. Scope of LC Four types of liquid chromatography -Partition - Adsorption (liquid-solid)
Hard Water. Clogged Pipes – Hard Water limestone hard water carbonic acid Step 1: Acid rain is formed Step 2: Acid rain dissolves limestone Water softener.
THE CHLOR-ALKALI INDUSTRY. Chlorine is manufactured by the electrolysis of brine. Sodium hydroxide is produced at the same time. Three different methods.
Acids Lesson 1 Acid and Base Properties. Taste sour Change litmus paper red React with metals such as Mg and Zn to make H 2 Are electrolytes that conduct.
HPLC 1. Introduction 1.Introduction CHROMATOGRAPHY Chromatography basically involves the separation of mixtures due to differences in the distribution.
An Introduction to Chromatography What is chromatography? The separation of a mixture by distribution of its components between a mobile and stationary.
Basic Chemistry Copyright © 2011 Pearson Education, Inc. 1 Chapter 14 Acids and Bases 14.9 Acid-Base Properties of Salt Solutions.
Created with MindGenius Business 2005® Instrumentation (1) Instrumentation (1) Origins Preparative column chromatography (Glass columns) Internal diameter:
© 2017 SlidePlayer.com Inc. All rights reserved.