1. Saline Tilapia (Oreochromis sp) Broodstock Production : Growth and Reproductive Performance as effort the seed supply for culture MOHAMAD SOLEH, AGUSTIN, KHAEMUDIN Main Center for Brackishwater Aquaculture (MCBA) (JEPARA-INDONESIA-2016

2. THE TILAPIAS : 1. Human global consume 2. Biofilter and commercial culture in pond 3. Potential development in sea water : floating net culture, live bait for pole and line fishing 4. High demand of saline offspring/seed for culture 5. Offspring must prepared from saline/salt tilapia breeder in order to appear the gen of high salinity tolerant THE TILAPIAS : 1. Human global consume 2. Biofilter and commercial culture in pond 3. Potential development in sea water : floating net culture, live bait for pole and line fishing 4. High demand of saline offspring/seed for culture 5. Offspring must prepared from saline/salt tilapia breeder in order to appear the gen of high salinity tolerant INTRODUCTION Reasons

3. 1. The consumers of tilapias have been globally (as : meat fillet : nice taste/delicious/unsoft, souvenir of it leather etc.) Fillet slipper, wallet shoes, belt

4. 2. Contributes as biofilter in shrimp culture to minimize the disease problems, but today become main species for commercial culture POLYCULTURE IN BRACKISH or SEA WATER POND (modern) TRADITIONAL CULTURE IN CAGE MONOCULTURE IN BRACKISH or SEA WATER POND (modern) COSTAL CULTURE The same pond with shrimp Mono culture, the water ca be continually exchange shrimp pond TRADITIONAL CULTURE IN LAND

5. OBJECTIVE : to produce saline tilapia breeder which have high salinity tolerant (> 30 g.L−1) by stress high salinity and have good performance during rearing in salt/sea water  TARGET : the breeder : optimum growth and spawn in water salinity of 10-15 g.L−1 OBJECTIVE and TARGET

6. - freshwater seed tilapia - length 3-5 cm/pcs - local strain of genetic improvement : GESIT/Sultana, Larasati, Kunti, Srikandi - artificial feed (commercial pellet, protein content > 25 %) - papaya extract (enzym) - concrete tank for rearing (water volume : 4 m 3 ), fibreglass/plastic box for high salinity stress (water volume : 100 l) - refractosalinometer, dissolve oxygen meter - timer/watch - field equipment - freshwater seed tilapia - length 3-5 cm/pcs - local strain of genetic improvement : GESIT/Sultana, Larasati, Kunti, Srikandi - artificial feed (commercial pellet, protein content > 25 %) - papaya extract (enzym) - concrete tank for rearing (water volume : 4 m 3 ), fibreglass/plastic box for high salinity stress (water volume : 100 l) - refractosalinometer, dissolve oxygen meter - timer/watch - field equipment MATERIALS & EQUIPMENTS

7. Freshwater offspring of genetic improvement adaptation (3-7 days) Direct stress to high salinity (35 g.L−1) stop SR 50% pop. or 2 hours stock density 1 pcs/l Stock in salinity 20 g.L−1, 24 hours Move to salinity > 30 g.L−1 Rearing the fingerling & broodstock in the sea water Spawning the breeder (10-20 g.L−1) Rearing offspring in the sea water Freshwater offspring of genetic improvement adaptation (3-7 days) Direct stress to high salinity (35 g.L−1) stop SR 50% pop. or 2 hours stock density 1 pcs/l Stock in salinity 20 g.L−1, 24 hours Move to salinity > 30 g.L−1 Rearing the fingerling & broodstock in the sea water Spawning the breeder (10-20 g.L−1) Rearing offspring in the sea water METHODE of SALINITY STRESS

8. Rearing in the tank, volume : 4 m3 Modular system, stocking density 250 – 300 pcs (weight 20 g/pcs); 200 – 250 pcs (weight 50 g/pcs); 150 – 200 pcs (weight 100 g/pcs); 100 – 150 pcs (weight 200 g/pcs) Commercial artificial feed (pellet, protein 25 %, 10-3 % biomass, freq. 3 times/day) Food enrichment by papaya extract : 50 ml/kg of feed, daily Siphoning debris (feces, uneating food etc.) Water salinity rearing : 30 g.L−1 (sea water) Water exchange > 50 %/day and circulated all day Rearing in the tank, volume : 4 m3 Modular system, stocking density 250 – 300 pcs (weight 20 g/pcs); 200 – 250 pcs (weight 50 g/pcs); 150 – 200 pcs (weight 100 g/pcs); 100 – 150 pcs (weight 200 g/pcs) Commercial artificial feed (pellet, protein 25 %, 10-3 % biomass, freq. 3 times/day) Food enrichment by papaya extract : 50 ml/kg of feed, daily Siphoning debris (feces, uneating food etc.) Water salinity rearing : 30 g.L−1 (sea water) Water exchange > 50 %/day and circulated all day Methods of REARING THE BREEDER

9. SPAWNING SYSTEM Tank preparation and water spawning (salinity 10- 15 g.L−1) Selection : sex (male-female), body weight each strains (GESIT/Sultana, Srikandi, Kunti, Larasati) Sex ratio 1 : 3 (male-female), stocking density 1 kg/m3 water Water exchange 50 % volume, a weekly Feeding : 1-2 % biomass/day, protein > 25 %, frequency 3 times a day Physically water quality control (temperature, dissolve oxygen) 2 times a week Eggs harvested last 30 days rearing or the larvae appear swim on water surface Tank preparation and water spawning (salinity 10- 15 g.L−1) Selection : sex (male-female), body weight each strains (GESIT/Sultana, Srikandi, Kunti, Larasati) Sex ratio 1 : 3 (male-female), stocking density 1 kg/m3 water Water exchange 50 % volume, a weekly Feeding : 1-2 % biomass/day, protein > 25 %, frequency 3 times a day Physically water quality control (temperature, dissolve oxygen) 2 times a week Eggs harvested last 30 days rearing or the larvae appear swim on water surface

10. Concrete tanks : facility for saline tilapias rearing (since fingerling to broodstock spawn)

11. Result stressing in high salinity (35 g.L−1) RED TILAPIABLACK TILAPIA Moribund fish Dead fish

12. Role defense of fry to stress treatment of high salinity (35 g.L−1) 15’ = fish live in bottom 30’ = water begin foam, fluid excretion, fish still in base 45’ = fish begin swimming in midle water 60’ = still swimming in midle water, apart swim in surface 75’ = same condition at 60’ 90’ = color of body line clear, fish swimming oblique 105’= more fish swimming on surface water 120’= more the fish swim on surface, bright color 150’= fish begin to dye, in the bottom tank, more in surface water 180’= dead fish increases in the bottom survival rate time

13. Sampling (S) Ʃ fish (pcs) SR (%) Biomass (kg) Length average (cm) Weight average (kg) Feeding daily (kg/%) Total feed (kg) Stock 189100.0 0.474 5.41±0.300.002 0.071 (15 %) 2.134 S1 18296.30 2.306 8.11±0.370.012 0.230 (10 %) 7.425 S2 15078.46 9.150 14.50±0.520.062 0.640 (7 %) 19.215 S3 13269.39 14.410 17.75±0.600.109 0.576 (4 %) 17.292 S4 116 61.10 18.807 18.27±0.56 0.152 -- 61.10 18.807 18.27±0.56 0.152 46.066 ADG Ave: 2.079 FCR1:2.454 Growth Performance of Gesit/Sultana strain (each 30 days)

14. Sampling (S) Ʃ fish (pcs) SR (%) Biomass (kg) Length average (cm) Weight average (kg) Feeding daily (kg/%) Total feed (kg) Stock 200100.00.62 4.75±0.310.003 0.093 (15 %) 2.812 S1 19898.402.30 8.93±0.400.008 0.230 (10 %) 6.927 S2 18487.703.55 11.59±0.540.022 0.248 (7 % ) 7.455 S3 16476.577.74 13.30±0.360.055 0.541 (7 % ) 16.254 S4 14366.649.19 16.58±0.700.074 0.643 (7 %) 19.299 S5 12361.5012.22 17.13±0.650.111 0.488 (4 %) 14.664 S6 11250.2313.75 18.02±0.900.144 - - Total 50.23 13.7518.02±0.900.14467.411 ADG Ave : 0.686 FCR1:4.757 Growth Performance of Larasati strain (each 30 days)

15. REPRODUCTIVE PERFORMANCE Eggs or larvae harvesting LarvaeLarvae with yolk Eggs or Larvae with yolk in the mouth Eggs

16. GESIT/Sultana breeder : stressed high salinity, spawned in brackishwater (salinity 10-15 g.L−1) Length (cm) Weight (g) Ʃ Eggs (pcs) Ʃ/g body weight Ʃ Larvae (pcs) Ʃ/g body weight 21.0 160 -- 800 (swim) 5.00 21.3 165 820 (swim) 4.96 20.0 130 -- 700 (swim) 5.38 22.5 225 -- 1,317 (eggs yolk) 5.85 18.5 110 -- 574 (eggs yolk) 5.21 18.2 105 -- 367 (eggs yolk) 3.49 21.7 230 -- 768 (eggs yolk) 3.33 21.5 200 -- 820 (eggs yolk) 4.10 22.0 235 -- 1,050 (swim) 4.47 20.5220 -- 1,000 (swim) 4.54 20.7210 -- 1,100 (swim) 5.23 19.5 125 -- 417 (swim) 3.25 19.0 120 -- 408 (swim) 3.40 13 samples Ave.= 4.47 (3.25-5.85)

17. GESIT/Sultana breeder : stressed high salinity, spawned in freshwater (0 g.L−1)……….. Length (cm) Weight (g) Ʃ eggs (pcs) Ʃ Egg/g body weight Ʃ Larvae (pcs) Ʃ/g body weight 21.0200--475 (swim)2.37 21.0200--559 (swim)2.79 20.01753502.00-- 21.0200--552 (eggs yolk)2.76 22.0225--766 (swim)3.40 19.2150--669 (swim)4.46 20.0150--535 (swim)3.56 21.0200--1,040 (swim)5.02 21.52001,1685.84-- 21.5200--500 (eggs yolk)2.50 24.0250--1,140 (eggs yolk)4.56 23.0200--600 (eggs yolk)3.00 22.01508705.80-- 23.0200--1,000 (swim)5.00

18. GESIT/Sultana breeder : stressed high salinity, spawned in freshwater (0 g.L−1)……….. Continued… Length (cm) Weight (g) Ʃ eggs (pcs) Ʃ egg/g body weight Ʃ Larvae (pcs) Ʃ/g body weight 22.0200--748 (swim)3.74 20.5200--740 (eggs yolk)3.70 21.2225 -- 886 (swim) 3.93 20.5150--615 (eggs yolk)4.10 26.0300 1,423 4.74-- 23.5300--1,710 (eggs yolk)5.70 26.0300--757 (eggs yolk) 2.52 24.0250--780 (swim) 3.12 21.0250--550 (swim) 2.20 Ave.= 3.60 23 samples (2.20-5.70)

19. Performance reproduction of KUNTI breeder stressed in high salinity, spawned in salinity 10-15 g.L−1 Length (cm) Weight (g) Ʃ Eggs (pcs) Ʃ egg/g body weight Ʃ Larvae (pcs) Ʃ/g body weight 21.5150 -- 783 (eggs yolk)5.22 22.02007423.71-- 19.0100 -- 224 (swim)2.24 20.5180 -- 1,000 (eggs yolk) 5.55 21.5200 -- 1,100 (swim)5.50 21.3200 -- 1,000 (swim)5.00 22.7 250 -- 1,100 (eggs yolk) 4.40 21.3 225 -- 945 (eggs yolk) 4.20 21.0 200 -- 645 (swim) 3.22 21.6210 -- 690 (swim) 3.28 22.5240 -- 862 (swim) 3.59 22.0300 -- 1,500 (eggs yolk) 5.00 21.7200 -- 500 (swim) 2.25 7423.71Ave.= 3.78

20. KUNTI breeder (spawned in salinity :10-15 g.L−1) ………… Length (cm) Weight (g) Ʃ Eggs (pcs) Ʃ egg/g body weight Ʃ larvae (pcs) Ʃ larvae/g body weight 21.0170 -- 950 (swim)5.58 22.52001,000 (egg yolk)5.00 20.0200 -- 1,645 (swim)8.22 21.0160 -- 1,050 (swim)6.56 19.5140 -- 786 (egg yolk)5.61 18.0100 -- 224 (egg yolk)2.24 22.5200 -- 1,364 (swim)6.82 20.0140 -- 950 (swim)6.77 20.5150 -- 530 (swim) 3.22 21.0162 -- 653 (eggs yolk) 3.28 23.0235 -- 1,030 (eggs yolk) 3.59 19.5138 -- 378 (eggs yolk) 5.00 22.0188 -- 1,100 (eggs yolk) 2.25 17.879227 (swim) 2.87 2,262 Total =11,887 Ave.=4.28 (2.24-8.22)

21. Performance reproduction of SRIKANDI breeder stressed in high salinity, spawned in salinity : 10-15 g.L−1 Length (cm) Weight (g) Ʃ Eggs (pcs) Ʃ Egg/g body weight Ʃ Larvae (pcs) Ʃ Larvae/g body weight 21.5300 -- 777 (swim)2.59 22.0280 -- 727 (egg yolk)2.60 22.5325-- 1,075 (swim) 3.30 21.8310-- 812 (swim) 2.63 22.5300 - - 538 (swim) 1.79 26.0400 - - 540 (swim) 1.36 23.0220 - - 1,632 (egg yolk)7.41 21.01501,0316.87-- 24.0250 - - 605 (swim)2.42 2,5356.786,706 Ave.= 3.03 total(1.36-7.41)

22. CONCLUSION To produce the broodstock of saline tilapia could be stressed to the seed (3-5 cm) in high salinity (35 g.L−1 ) and always in the sea water media during reared (salinity 30 g.L−1 or more) Saline tilapia of Gesit/Sultana strain (black tilapia) performed fast growth, short the time of culture and more high of ADG, FCR and survival rate than Larasati strain (red tilapia) In this trial females of saline tilapia of Gesit/Sultana breeder was spawned in salinity of 10-15 g.L−1 performed better larvae produces (4.47 pcs/g body weight) than Kunti (4.28 pcs) and Srikandi (3.03 pcs) Comparative spawned of saline tilapia of Gesit/Sultana breeder in water salinity of 10-15 g.L−1 performed larvae produces 4.47 pcs/g body weight is more better than in freshwater (3.60 pcs/g body weight) To produce the broodstock of saline tilapia could be stressed to the seed (3-5 cm) in high salinity (35 g.L−1 ) and always in the sea water media during reared (salinity 30 g.L−1 or more) Saline tilapia of Gesit/Sultana strain (black tilapia) performed fast growth, short the time of culture and more high of ADG, FCR and survival rate than Larasati strain (red tilapia) In this trial females of saline tilapia of Gesit/Sultana breeder was spawned in salinity of 10-15 g.L−1 performed better larvae produces (4.47 pcs/g body weight) than Kunti (4.28 pcs) and Srikandi (3.03 pcs) Comparative spawned of saline tilapia of Gesit/Sultana breeder in water salinity of 10-15 g.L−1 performed larvae produces 4.47 pcs/g body weight is more better than in freshwater (3.60 pcs/g body weight)

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