1. IDENTIFICATION OF ENTEROBACTERIACEAE
Islamic University of Gaza
Faculty of science
Medical technology department
الجامعة الاسلامية بغزه
كلية العلوم
قسم التحاليل الطبية
IDENTIFICATION OF ENTEROBACTERIACEAE
Prepared by:
Abdullah Ali
Supervised by:
Dr.Abdellraof el-Manama
Dr.Nedal Fayyad

2. Characteristics of Enterobacteriaceae
Members of the Enterobacteriaceae are Gram-negative, straight rods, some of which are motile. Most species grow well at 37°C,
although some species grow better at °C. They are facultatively
anaerobic, oxidase-negative and catalase-positive (except Shigella dysenteriae type 1). They are distributed worldwide and may be
found in soil, water, plants and animals.

3. Common genera of the family Enterobacteriaceae
7) Morganella morganii
8) Proteus species
9) Providencia species
10) Salmonella species
11) Serratia species
12) Yersinia species
1) Citrobacter species
2) Enterobacter species
3) Escherichia species
4) Hafnia alvei
5) Klebsiella species
6) Shigella species

4. Incidence of Enterobacteriaceae Associated with Bacteremia

6. Escherichia
Escherichia coli

7. Salmonella

8. Enterobacter
Enterobacter aurogeouns
Enterobacter sakazakii

9. Shigella

10. Citrobacter

11. Can they ferment lactose? Can they ferment lactose?
(No)
(Yes)
Can they use citric acid as their sole carbon source?
Can they use citric acid as their sole carbon source?
Can they use citric acid as their sole carbon source?
(No)
(Yes)
(No)
(Yes)
Do they produce acetoin?
Salmonella
Escherichia
Shigella
(No)
(Yes)
Citrobacter
Enterobacter

12. Tests:
From the previous slide we observe that we need to do the following tests:
1)Lactose fermentation
2)Citrate test
3)Voges-proskauer test

14. Citrate
test

16. Some disease causing by Enterobacteriaceae species
.diarrhea, invasive disease
E. coli
diarrhea, septicemia, pneumonia
Salmonella
.dysentery
Shigella

17. Conclusion Family Enterobacteriaceae have the following
characteristic:
1)Gram-negative rod
2)Non-sporeforming bacteria
3)Facultative anaerobe
4)Motile by peritritchous flagella
5)Oxidase negative
6)Non acid fast bacteria
7)Produce nitrate reductase

18. Thanks for your attention