2 Citrobacter Resembles Salmonella but are ONPG (+) & LDC (-) Citrobacter speciesfreundii (UTI, pneumonia, intraabdominal abscess)diversus (neonatal meningitis)amalonaticus (extraintestinal infections)C. freundii S. diversusH2SIndoleKCN
3 KlebsiellaKlebsiella -Enterobacter-Serratia-Hafnia K. pneumoniae >Friedlander’s bacilli (encapsulated and mucoid) > (+) String test Klebsiella aerogenes is associated with hospital acquired infections of wounds and of the urinary tract K. rhinoschleromatis – rhinoscleromatis (granulomatous disease of the nose and pharynx with intense swelling and malformation of the entire face and neck)
4 The patient's K. pneumoniae isolate was string test positive (>5 mm string length).
5 LABORATORY DIAGNOSESSpecimens: Depending on the site of infection, specimensinclude urine, pus, sputum and infected tissue.MorphologyKlebsiellae are Gram negative, non-motile, usuallycapsulated rods.CultureKlebsiellae are aerobes and facultative anaerobes.Blood agar: Klebsiellae produce large grey white usually mucoid colonies.MacConkey agar and CLED medium: Most klebsiellae are lactose-fermenting, producing mucoid pink colonies on MacConkey agar and yellow mucoid colonies on CLED mediumK. rhinoscleromatis is non-lactose fermenting.Biochemical testsKlebsiellae are indole negative, ornithine decarboxylase negative and do not produce H2S.
6 Klebsiella on CLED medium show yellow lactose fermenting colony Klebsiella on Macconkey medium show large mucoid pink lactose fermenting colony
7 Enterobacter -species: habitat: soil, water, dairy products -normal flora of the gut of animals & humans-motile, ODC & ONPG (+)LDC ADH ODC Urease YellowpigmentE. cloacae V -E. aerogenesE. gergoviaeE. sakazakiiE. taylorae
12 Proteus Tow important species , P. mirabilis and P. vulgaris MorphologyProteus is actively motile with peritricous flagella , non capsulate, Gram negative pleomorphic rods.Rapid urease producersSwarming, burned chocolate odor
14 Pathogenicity P. mirabilis causes: 1- Urinary infections. in the elderly and young males and often following catheterization or cystoscopy..2- Abdominal and wound infections3- secondary invader of ulcers, burns and damaged tissues.4- Septicaemia5- Meningitis and Chest infections.
15 is electrolyte deficient Biochemical tests Cultureaerobically, most Proteus cultures have a characteristic ‘fishy’ Odour and Swarming on the mediumMacConkey, CLED, XLD media: non-lactose fermenting colonies after overnight incubation at 35–37 ºCSwarming is prevented on MacConkey agar and XLD agar because these media contain bile salts.Swarming is inhibited on CLED agar because itis electrolyte deficientBiochemical testsDo not ferment lactose.Rapidly hydrolyze urea (within 4 hours), early screening test in differentiating enteric pathogens, e.g. salmonellae and shigellae from Proteus.Phenylalanine deaminase (PDA), positive.Indole negative (P. vulgaris is indole positive).
16 Proteus P. mirabilis P. vulgaris Indole - + ODC + - Fermentation MaltoseXyloseSalicinChloramphenicol S R
17 Proteus P. mirabilis P. vulgaris Indole ……… ……. Maltose ……… ……. Xylose …….. …….Salicin …….. …….Chloramphenicol …….. ……
24 Revision Question1- Characteristic of enterobacterceae all of the following except :a- Gram negative rodb- Facultative anaerobicc- Ferment glucosed- Oxidase positive2-Sworming is special characteristic of:a. Pseudomonadesb- Proteusc- E. colid- Klebsiella3- Mucoid colony is special characteristic of:
25 4- Bacteria which give green metallic sheen on EMB medium is: a. Pseudomonadesb- Proteusc- E. colid- Klebsiella5- Polysaccharide capsule present on which of the following bacteria:c- Shigella6- Which of the following bacteria is urease positive :7- Which of the following bacteria is lactose fermenter:
26 When we inoculate members of enterobacterceae on MacConkey agar, mention which organism grow producing lactose fermentor colony and which grow producing lactose fermentor colony
27 الهم لا تجعل الدنيا اكبر همنا و لا مبلغ علمنا klebsiella and e.coliالهم لا تجعل الدنيا اكبر همنا و لا مبلغ علمنا