1. Introduction to Basic Science Emily L. Lowe, Ph.D. Microbiology, Immunology and Molecular Genetics UCLA

2. Outline What is “The Scientific Method”? What is the difference between basic scientific research and clinical research? What is translational research? What are the key basic scientific questions remaining for HIV researchers?

3. New Questions Arise Results Interpreted Data Collected Question Identified Hypotheses Formed Research Plan The Basic Scientific Method If…then… Share Your Data

4. The Basic Scientific Method: Identifying the Problem First, and arguably the most important, step – Several sources Theoretical basis Professional practice Personal experience Shear curiosity – Starts as a broad question that must be narrowed – Problem statement; experimental approach to the problem; etc. Question Identified

5. The Basic Scientific Method: Formulating a Hypothesis Question Identified Hypotheses Formed If…then…

6. Hypothesis: – A belief or prediction of the eventual outcome of the research – A concrete, specific statement about the relationships between phenomena – Must be falsifiable – 2 types of hypotheses: Null hypothesis – All is equal; no differences exist Alternative (research) hypothesis – Usually specific and opposite to the null The Basic Scientific Method: Formulating a Hypothesis If…then…

7. The goal of basic science is to challenge existing paradigms and dogma!!!

8. The Basic Scientific Method: Developing the Research Plan Question Identified Hypotheses Formed Research Plan If…then…

9. A strategy must be developed for gathering and analyzing the information that is required to test the hypotheses or answer the research question – Four parts: Selection of a relevant research methodology Identification of cell types, samples or subjects Description of the data-gathering procedures Specification of the data analysis techniques – Pilot studies must be determined in advance The Basic Scientific Method: Developing the Research Plan

10. The Basic Scientific Method: Collecting and Analyzing the Data Data Collected Question Identified Hypotheses Formed Research Plan If…then…

11. Following all the pre-determined protocols – Time in the lab collecting data – Controlling the environment – Analyzing the composite data The Basic Scientific Method: Collecting and Analyzing the Data

12. The Basic Scientific Method: Interpreting Results and Forming Conclusions Results Interpreted Data Collected Question Identified Hypotheses Formed Research Plan If…then… New Questions Arise

13. Does the evidence support or refute the original hypotheses? – Accept or reject the hypotheses – Conclusions should be drawn: Develop new hypotheses to explain the results Data analysis is not the end! The Basic Scientific Method: Interpreting Results and Forming Conclusions

14. New Questions Arise Results Interpreted Data Collected Question Identified Hypotheses Formed Research Plan The Basic Scientific Method If…then… Share Your Data

15. Clinical Research Research conducted on humans or human tissues that makes use of patient data. Includes study of disease mechanisms, therapeutic interventions, epidemiology, and clinical trials. Aimed at understanding human disease and improving human health. Interaction between researchers and patient data is a key feature. Basic Scientific Research Fundamental theoretical or experimental investigative research to advance knowledge without a specifically envisaged or immediately practical application. It is the quest for new knowledge and the exploration of the unknown. Takes many years for the results of basic research to find some practical utility.

16. Basic and Clinical Research are Interdependent Translation from basic science to human studies Translation of new knowledge into clinical practice Basic Research Clinical Research Improved Health Adapted from Sung et al. (2003) JAMA, 289, 1278-89. Copyright © (2003) American Medical Association. All Rights reserved.

17. Translational Research Differs from Basic Scientific Research Basic Scientific Motivation: curiosity Goal: new knowledge – No matter the result (proven or disproven the hypothesis) new knowledge is obtained Translational Motivation: to improve human health Goal: better health

18. C. Simone Fishburn. Translational research: the changing landscape of drug discovery. Drug Discovery Today Volume 18, Issues 9–10 2013 487 – 494 Basic and Clinical Research Meet at Translational Research

19. Questions Still Remaining for Basic Scientific Research What are the molecular interactions involved in the regulation of HIV expression and replication in human immune cells? Why is the host immune response not fully effective in controlling the infection? How do reservoirs of infection persist in the body despite highly active antiretroviral treatment (HAART)?