1. THE PROKARYOTES

2. Systematics of Prokaryotes Focus on animals and plants –History limited to 20% of evolutionary time How to classify prokaryotes? Limited in morphological characters

3. Carl Richard Woese 1928-2012, USA; Developed system based on 16S rRNA in 1977

4. Carl Woese and George Fox

5. rRNA Zuckerkandl and Pauling Emile Zuckerkandl (1922-2013); Austria & USA. Molecular biology and molecular clock Linus Carl Pauling (1901-1994) USA Founder of fields like quantum chemistry and molecular biology Suggested that a tree of life might be generated by comparing sequences of biopolymers like RNA

6. Why should rRNA be conserved?

7. When DNA is transcribed, the result is an RNA molecule Figure 10.10 DNA molecule Translation Polypeptide Gene 1 Gene 2 Gene 3 DNA strand Transcription RNA Codon Amino acid

8. When DNA is transcribed, the result is an RNA molecule RNA is then translated into a sequence of amino acids Figure 10.10 DNA molecule Translation Polypeptide Gene 1 Gene 2 Gene 3 DNA strand Transcription RNA Codon Amino acid

9. Ribosomal Function A typical prokaryotic cell may have 10,000+ ribosomes

10. Where does rRNA enter the picture?

11. Ribosomal Structure Two subunits

12. Ribosomal subunits= rRNA molecules + proteins

13. Prokaryotes Eukaryotes

14. What’s the ‘S’? Svedberg units: a measure of how quickly particles sediment in an ultracentrifuge

15. What’s the ‘S’? Svedberg units: a measure of how quickly particles sediment in an ultracentrifuge Larger the particle, the greater its S value Smaller subunit of a ribosome sinks slower than the larger subunit

16. Why then does 5S + 23S = 50S?

17. Why then does 5S + 23S = 50S? Shape AND size determine sedimentation rate…

18. Ribosomal RNA Molecules Components of the ribosomes of ALL ORGANISMS Changes in rRNA nucleotide sequence indicative of evolutionary history SSU rRNA

19. A modification of Woese from Brock et al. (1994).

20. Two different supertrees generated by ML methods for complete genomes of 45 taxa. Daubin et al. 2002

21. Ciniglia et al. 2004

22. Lang et al. 2013 Using 24 genes and 3000 taxa

23. Gram Stain and Structure

24. Eubacteria  >9 Kingdoms  Same type of ribosomes  Polysaccharide of outer wall made of Murein  Most groups involved in global nutrient cycling  Many of economic importance  Disease  Other functions (e.g. antibiotic producers)

25. Archaea Differ from the Eubacteria –Form of ribosomes –No murein –Different lipids –Different RNA polymerase