2. BLOOD

4. William Harvey- father of physiology: discovered that blood circulated through the body in 1628.

6. Blood..a liquid “tissue”??? Blood..a liquid “tissue”???

7. How are Tissue Classified ?(AGAIN) Epithelial- lining or covering and glandular tissue. Muscle- highly specialized to contract Nervous- irritability and conductivity Connective- Connects body parts; Must be living cells surrounded by a matrix. Example: Bone (cells sitting in lacunae in hardened matrix of calcium salts in Collagen fibers)

8. Blood is considered to be a type of connective tissue. Even though it has a different function in comparison to other connective tissues it does have an extracellular matrix. Blood is considered to be a type of connective tissue. Even though it has a different function in comparison to other connective tissues it does have an extracellular matrix. The matrix is the plasma and erythrocytes, leukocytes and platelets are suspended in the plasma. The matrix is the plasma and erythrocytes, leukocytes and platelets are suspended in the plasma.

9. Blood The only fluid tissue in the human body The only fluid tissue in the human body Classified as a connective tissue Classified as a connective tissue Living cells = formed elements Living cells = formed elements Non-living matrix = plasma Non-living matrix = plasma

10. Introduction Blood, a type of connective tissue, is a complex mixture of cells, chemicals, and fluid. Blood transports substances throughout the body, and helps to maintain a stable internal environment. The blood includes Red blood cells – oxygen carrying cells White blood cells – fights infections Platelets – assist in the clotting of blood Plasma – water, proteins, vitamins, cellular wastes, etc. A blood hematocrit is normally 45% cells and 55% plasma.

12. Red Blood Cells Red blood cells (erythrocytes) are biconcave disks that contain one-third oxygen-carrying hemoglobin by volume. When oxygen combines with hemoglobin bright red oxyhemoglobin results. Deoxygenated blood (deoxyhemoglobin) is darker. Red blood cells discard their nuclei during development and so cannot reproduce or produce proteins. Typical Red Blood cell counts (Approx. 5 million per drop) 4,600,000 - 6,200,000 cells per mm 3 for males 4,500,000 - 5,100,000 cells per mm 3 for females. The number of red blood cells is a measure of the blood's oxygen carrying capacity.

14. The average life span of a red blood cell is 120 days. The number of red cells is held relatively constant by the hormone erythropoietin (produced by kidneys)

15. Control of Erythrocyte Production Rate is controlled by a hormone (erythropoietin) Rate is controlled by a hormone (erythropoietin) Kidneys produce most erythropoietin as a response to reduced oxygen levels in the blood Kidneys produce most erythropoietin as a response to reduced oxygen levels in the blood Homeostasis is maintained by negative feedback from blood oxygen levels Homeostasis is maintained by negative feedback from blood oxygen levels

16. Control of Erythrocyte Production: Homeostasis Figure 10.5

17. Hypoxia Prolonged oxygen deficiency due to deoxyhemoglobin (red blood cells not binded to O2) leads to cyanosis Prolonged oxygen deficiency due to deoxyhemoglobin (red blood cells not binded to O2) leads to cyanosis

18. White Blood Cells White blood cells (leukocytes) help defend the body against disease. Normally a milliliter of blood contains 5,000 to 10,000 white blood cells. There are five types of white cells that can be distinguished by physical features. 1.Neutrophils have fine cytoplasmic granules and a multi-lobed nucleus when red staining They comprise 54-62% of leukocytes. Ingest and destroy bacteria 2.Eosinophils have coarse granules that stain deep red, a bi- lobed nucleus, 1-3% of circulating leukocytes. Control allergic reactions by removing chemicals

19. Neutrophil – multi- lobed nucleus. Fine cytoplasmic granules Eosinophil –cytoplasmic granules when red stained

20. 3. Basophils have fewer granules that stain blue Fewer than 1% of leukocytes. Release heparin and histamine, which increases blood flow Cause body ’ s response to allergens.

21. 4.Monocytes are the largest blood cells, have variably shaped nuclei Make up 3-9% of circulating leukocytes. Can engulf and kill larger invading objects 5.Lymphocytes are long-lived, have a large, round nucleus, 25-33% of circulating leukocytes. Important for production of antibodies.

22. Monocyte – kidney, oval, or lobe shaped nucleus. Lymphocyte – large round nucleus.

24. Blood Plasma Plasma is the clear, straw-colored fluid portion of the blood. Plasma is mostly water (90+%) but contains a variety of substances. Plasma functions to transport nutrients and gases, regulate fluid and electrolyte balance, and maintain a favorable pH. Plasma proteins fall into three groups 1.The albumins help maintain the osmotic pressure of the blood and account for 60% of the plasma proteins. 2.The globulins, comprising 36% of the plasma proteins, are designated as alpha, beta, and gamma globulins. Alpha and beta globulins transport lipids and fat soluble vitamins Gamma globulins are a type of antibody 3.Fibrinogen (4%) plays a primary role in blood coagulation.

25. Plasma also carries oxygen and carbon dioxide. Oxygen is carried in low levels in the plasma but mostly by the red blood cells. Carbon dioxide is carried in the plasma as carbonic acid. Additionally, there are molecules called lipoproteins that help transport lipids throughout the bloodstream Chylomicrons – transport dietary fats from small intestine VLDL – transport triglycerides from liver to adipose tissue LDL – Delivers cholesterol to cells (Considered to be “ Bad ” cholesterol.) HDL – transports broken down chylomicrons to liver. (Considered to be “ Good ” cholesterol.)

26. Hemostasis Hemostasis refers to the stoppage of bleeding. Following injury to a vessel, three steps occur in hemostasis: 1.Blood vessel spasm Roughly cutting a blood vessel causes the muscle in its walls to contract in a reflex, or engage in vasospasm. 2.Platelet plug formation Platelets stick to the exposed edges of damaged blood vessels, forming a net. A platelet plug is most effective on a small vessel. 3.Blood coagulation A variety of chemicals and proteins are used to form a clot

30. ABO Blood Group This refers to the type of antigens and antibodies found in the blood stream. It allows doctors to determine what type of blood to give if a transfusion is necessary. The wrong blood can cause agglutination in a patient leading to death. Antigens – molecules on the outer surface of the cell Antibodies – molecules floating in plasma that help fight infections. Type O is most common (47%) and has no antigens but both A and B antibodies Type A is next (41%) and has A antigens and B antibodies. Type B follows (9%) and has B antigens and A antibodies. Type AB is least common (3%) and has A and B antigens and no antibodies.

33. PhenotypesGenotypes Type AAA, Ao Type BBB, Bo Type ABAB Type Ooo

34. Was Charlie Chaplin the father? In 1944 the actress Joan Barry (blood group A) sued Charlie Chaplin (blood group O) for support of her child (phenotype B). The blood group evidence was presented in court. The jury supported Joan Barry’s claim. Were they right? The mother must be Heterozygous A (Ao) The child must be Heterozygous B (Bo, inheriting the “o” from mother) The child must have inherited a “B” from the father. Charlie Chaplin was Homozygous O (oo) and therefore could not have been the father. ?

35. The Rh factor on blood refers to the positive or negative part of the blood type. Rh+ blood has an extra molecule on the blood cell. Rh- blood does not have this extra molecule. It can lead to problems like erythroblastosis fetalis Occurs when an Rh- woman and an Rh+ man conceive a child.