1. Shifting meiotic to mitotic spindle assembly in oocytes disrupts chromosome alignment
ASpinning disk confocal microscopy images showing spindle region magnifications of oocytes expressing GFP‐EB3 (green), mCherry‐Plk4 (red), and HSET WT at NEBD+1h30, +4h30, and +6h30. Scale bar 10 μm. BQuantification in 3D of spindle length in oocytes expressing HSET WT (blue dots) at NEBD+1h30, +4h30, and +6h30. Each dot represents an oocyte; the number of oocytes analyzed is written in parentheses. Statistical significance of differences is assessed with a Mann–Whitney test: *P‐value = CSpinning disk confocal microscopy images showing spindle region magnifications of oocytes expressing GFP‐EB3 (green) and mCherry‐Plk4 (red) together with HSET WT (HSET WT, right panels) or not (Ctrl, left panels) at NEBD+1h30 and NEBD+6h30. Scale bar 10 μm. D–FQuantification in 3D of spindle length (D) *P‐value = 0.022, ***P‐value < ; spindle pole width (E) *P‐value = 0.046, ***P‐value < and central spindle width (F) *P‐value = 0.037, ***P‐value < in oocytes overexpressing HSET WT (blue dots) and controls (gray dots) at NEBD+1h30 and NEBD+7h. Each dot represents an oocyte; the number of oocytes analyzed is written in parentheses. Statistical significance of differences is assessed with a Mann–Whitney test.
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EMBO Rep, Volume: 19, Issue: 2, Pages: , First published: 12 January 2018, DOI: ( /embr )