1. The trimeric coiled‐coil HSBP1 protein promotes WASH complex assembly at centrosomes
MDA‐MB‐231 cells expressing HaloTag WASH or CCDC53 were stained with the fluorescent TMR ligand, HSBP1, and γ‐tubulin antibodies and DAPI to stain nuclei. WASH and CCDC53 colocalize with HSBP1 at the centrosome. HSBP1 is also associated with centrosomes in tissue sections from human breast. The HSBP1 staining is more elongated in shape in normal tissue than in mammary carcinomas. Seven biopsies of breast tumors displaying both carcinoma and adjacent normal tissue were tested, and they all gave similar staining. Scale bars: 10 μm.
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EMBO J, Volume: 37, Issue: 13, First published: 29 May 2018, DOI: ( /embj )