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Co‐immunoprecipitation of Larp with MDI‐myc in transfected S2 cells

Published byArchibald Ellis Modified over 4 years ago

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Presentation on theme: "Co‐immunoprecipitation of Larp with MDI‐myc in transfected S2 cells"— Presentation transcript:

1 The mitochondrial outer membrane protein MDI promotes local protein synthesis and mtDNA replication
Co‐immunoprecipitation of Larp with MDI‐myc in transfected S2 cells. Tubulin was used as a loading control. mtDNA replication, as illustrated by EdU incorporation, in wt and larp mutant (larp) ovarioles. mtDNA replication is dramatically reduced in the larp ovariole. Arrows: mtDNA; arrowheads: nuclei. wt and mdi1 egg chambers stained for Larp (green) and ATP‐S (red) to reveal mitochondria. Larp closely associates with mitochondria in wt egg chambers. Mitochondria in mdi1 flies are clumped together and completely lack Larp staining. Data information: Scale bars in (B, C), 10 μm. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 35, Issue: 10, Pages: , First published: 06 April 2016, DOI: ( /embj )

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