1. KLF4 is a key determinant in the development and progression of cerebral cavernous malformations
qRT–PCR analysis of Klf4 during disease progression at different times (P3, P5, and P9) after tamoxifen‐induced Ccm1 recombination (P1) in freshly isolated brain ECs derived from WT and iCCM1 mice. Data are mean ± SD (n = 3 in each group). Fold changes are relative to WT animals. A two‐tailed unpaired t‐test was performed. *P = 0.0143, **P = 0.002, ##P =  Representative immunostaining of KLF4 (red) in combination with PECAM1 (green, to identify ECs) in brain sections from iCCM2, iCCM3 mice, and their relative WT controls (one out of three performed). Cell nuclei are visualized with DAPI; dotted area highlights lesion area. Scale bars: 50 μm.
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EMBO Mol Med, Volume: 8, Issue: 1, Pages: 6-24, First published: 26 November 2015, DOI: ( /emmm )