1. Absolute quantification of translational regulation and burden using combined sequencing approaches
Genetic design of the LacZ reporter construct whose expression is activated by the inducer IPTG. Normalized RPF count profile averaged for all E. coli transcripts. Profiles generated for cells grown in the absence and presence of IPTG (1 mM). Start and stop codons are shaded. Bar chart of all measured RBS initiation rates ranked by their strength. Strong RBSs with initiation rates > 1 ribosome/s are highlighted in red. Bar chart of all measured translation termination efficiencies at stop codons ranked by their strength. Stop codons with translation termination efficiency > 0.99 are highlighted in red. Distribution of initiation rates for cells grown in the absence and presence of IPTG (1 mM). Distribution of translation termination efficiencies for cells grown in the absence and presence of IPTG (1 mM).
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Mol Syst Biol, Volume: 15, Issue: 5, First published: 03 May 2019, DOI: ( /msb )