Presentation is loading. Please wait.

Presentation is loading. Please wait.

Martin Depner, PhD, Markus J. Ege, MD, Michael J

Published byLesley Rosanna Davis Modified over 5 years ago

Similar presentations

Presentation on theme: "Martin Depner, PhD, Markus J. Ege, MD, Michael J"— Presentation transcript:

1 Bacterial microbiota of the upper respiratory tract and childhood asthma 
Martin Depner, PhD, Markus J. Ege, MD, Michael J. Cox, PhD, Sarah Dwyer, PhD, Alan W. Walker, PhD, Lena T. Birzele, Jon Genuneit, MD, Elisabeth Horak, MD, Charlotte Braun-Fahrländer, MD, Hanna Danielewicz, MD, PhD, Raina M. Maier, PhD, Miriam F. Moffatt, PhD, William O. Cookson, MD, Dick Heederik, PhD, Erika von Mutius, MD, Antje Legatzki, PhD  Journal of Allergy and Clinical Immunology  Volume 139, Issue 3, Pages e13 (March 2017) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Sampling sites and timing. A, Anatomic location of the throat (T) and nasal (N) sampling site. B, Distribution of sampling time points of the nasal and throat samples. Methodical differences in sample processing preclude direct comparison of throat and nasal samples. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Multiple rarefaction curves for richness (A) and Shannon index (B) for all 327 throat (T) and 68 nasal (N) samples, respectively. Shannon index is shown up to a rarefaction of only 9100 sequences per sample. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Taxonomic composition of the microbiota from throat and nose. Survey-weighted relative abundance of phyla (A) and genera (B) for throat (T) and nasal (N) samples. Genera belonging to the same phylum are represented by the color of the respective phylum. Phyla and genera representing less than 0.5% of all reads for phyla and less than 1% for genera in the combined throat and nasal sample, respectively, are grouped in “rare.” Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Bacterial diversity in throat (T) and nasal (N) samples from children with asthma and controls. Shown are β-diversity as unweighted UniFrac (A), and α-diversity as richness (B) and Shannon index (C) for throat samples and nasal samples. The P values for Wilcoxon test comparing asthma versus control group are shown. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Relative abundance of Moraxella OTU 1462 in children with asthma and controls stratified for farming. The P value refers to the association with asthma in the respective stratum. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 Inverse correlation of richness and relative abundance of Moraxella OTU 1462 in nasal samples stratified for farming. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E1 Study design. Shown are the final throat (N = 327) and nasal (N = 68) study populations and their derivations. The recruitment population consisting of children with asthma and control children from the Bavarian GABRIELA study population was the origin of the 2 analysis populations. For 4 children of the recruitment population, no asthma information was available (*). Stratified random sampling was used. N is the number of children for the different groups in the respective population. Note that samples with low read counts after sequencing and filtering (<1000 reads) were removed in the final study population. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig E2 Number of sequences per sample for the 3 analysis populations after denoising and filtering. At the right border, sum, mean, and SD of sequences are reported. Methodical differences in sample processing preclude direct comparison of throat and nasal samples. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Fig E3 Taxonomic composition of the throat microbiota based on 327 and 68 children, respectively. The lower panel is restricted to the throat samples of the 68 children who also provided nasal samples. Shown is the survey-weighted relative abundance of phyla (A) and genera (B). Genera belonging to the same phylum are represented by the color of the respective phylum. Phyla and genera representing less than 0.5% of all reads for phyla and less than 1% for genera in the combined throat and nasal sample, respectively, are grouped in “rare.” Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

11 Fig E4 Taxonomic composition and richness stratified for age, sampling month, and clinical conditions. Shown is the relative abundance of genera (A) and the boxplots for richness (B) in throat (T) and nasal (N) samples, respectively. The P values of Wilcoxon or Kruskal-Wallis tests refer to comparisons across strata. All values refer to unweighted analyses. Methodical differences in sample processing preclude direct comparison of throat and nasal samples. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

12 Fig E5 Number of bacterial 16S rRNA gene copies in throat and nasal samples. The analyses are based on 327 throat and 68 nasal samples, grouped by asthma occurrence and farm exposure. The P values of survey-weighted Wilcoxon tests refer to comparisons between asthma cases and controls. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

13 Fig E6 Bacterial diversity in throat (T) and nasal (N) samples from children with asthma and controls based on samples with adequate reads. Shown are β-diversity as unweighted UniFrac (A), and α-diversity as richness (B) and Shannon index (C) for throat samples and nasal samples. Adequate read counts were defined by the median of reads, that is, 4635 reads for throat samples and 8120 reads for nasal samples. The P values for Wilcoxon tests comparing asthma versus control group are shown. Journal of Allergy and Clinical Immunology  , e13DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Martin Depner, PhD, Markus J. Ege, MD, Michael J"

Similar presentations

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,

Early exposure to cow's milk protein is protective against IgE-mediated cow's milk protein allergy Yitzhak Katz, MD, Nelly Rajuan, MSc, Michael R. Goldberg,
Effects of early-life exposure to allergens and bacteria on recurrent wheeze and atopy in urban children  Susan V. Lynch, PhD, Robert A. Wood, MD, Homer.

Effects of early-life exposure to allergens and bacteria on recurrent wheeze and atopy in urban children  Susan V. Lynch, PhD, Robert A. Wood, MD, Homer.
Vitamin D over the first decade and susceptibility to childhood allergy and asthma  Elysia M. Hollams, PhD, Shu Mei Teo, PhD, Merci Kusel, MBBS, PhD, Barbara.

Vitamin D over the first decade and susceptibility to childhood allergy and asthma  Elysia M. Hollams, PhD, Shu Mei Teo, PhD, Merci Kusel, MBBS, PhD, Barbara.
Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 
Association of physical activity, asthma, and allergies: A cohort of farming and nonfarming children  Bettina Bringolf-Isler, MD, PhD, Eva Graf, MD, Marco.

Association of physical activity, asthma, and allergies: A cohort of farming and nonfarming children  Bettina Bringolf-Isler, MD, PhD, Eva Graf, MD, Marco.
Latent class analysis reveals clinically relevant atopy phenotypes in 2 birth cohorts  Alexander J. Hose, MA, MPH, Martin Depner, PhD, Sabina Illi, PhD,

Latent class analysis reveals clinically relevant atopy phenotypes in 2 birth cohorts  Alexander J. Hose, MA, MPH, Martin Depner, PhD, Sabina Illi, PhD,
Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications  Elena Goleva,

Airway microbiome and responses to corticosteroids in corticosteroid-resistant asthma patients treated with acid suppression medications  Elena Goleva,
Eosinophilic airway inflammation in asthmatic patients is associated with an altered airway microbiome  Asger Sverrild, MD, PhD, Pia Kiilerich, MSc, PhD,

Eosinophilic airway inflammation in asthmatic patients is associated with an altered airway microbiome  Asger Sverrild, MD, PhD, Pia Kiilerich, MSc, PhD,
Inflammatory phenotypes in patients with severe asthma are associated with distinct airway microbiology  Steven L. Taylor, BSc, Lex E.X. Leong, PhD, Jocelyn.

Inflammatory phenotypes in patients with severe asthma are associated with distinct airway microbiology  Steven L. Taylor, BSc, Lex E.X. Leong, PhD, Jocelyn.
Effects of early-life exposure to allergens and bacteria on recurrent wheeze and atopy in urban children  Susan V. Lynch, PhD, Robert A. Wood, MD, Homer.

Effects of early-life exposure to allergens and bacteria on recurrent wheeze and atopy in urban children  Susan V. Lynch, PhD, Robert A. Wood, MD, Homer.
Prenatal and early-life exposures alter expression of innate immunity genes: The PASTURE cohort study  Georg Loss, MSc, Sondhja Bitter, MD, Johanna Wohlgensinger,

Prenatal and early-life exposures alter expression of innate immunity genes: The PASTURE cohort study  Georg Loss, MSc, Sondhja Bitter, MD, Johanna Wohlgensinger,
Georg Loss, PhD, Martin Depner, PhD, Laurien H. Ulfman, PhD, R. J

Georg Loss, PhD, Martin Depner, PhD, Laurien H. Ulfman, PhD, R. J
Increased regulatory T-cell numbers are associated with farm milk exposure and lower atopic sensitization and asthma in childhood  Anna Lluis, PhD, Martin.

Increased regulatory T-cell numbers are associated with farm milk exposure and lower atopic sensitization and asthma in childhood  Anna Lluis, PhD, Martin.
Markus J. Ege, MD, David P. Strachan, MD, William O. C. M

Markus J. Ege, MD, David P. Strachan, MD, William O. C. M
Atopic sensitization in the first year of life

Atopic sensitization in the first year of life
Antti E. Seppo, PhD, Chloe A. Autran, MSc, Lars Bode, PhD, Kirsi M

Antti E. Seppo, PhD, Chloe A. Autran, MSc, Lars Bode, PhD, Kirsi M
Effect of probiotics in prevention of atopic dermatitis is dependent on the intrinsic microbiota at early infancy  Ekaterina Avershina, PhD, Raul Cabrera.

Effect of probiotics in prevention of atopic dermatitis is dependent on the intrinsic microbiota at early infancy  Ekaterina Avershina, PhD, Raul Cabrera.
Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD 
Joe K. Gerald, MD, PhD, Roni Grad, MD, William C. Bailey, MD, Lynn B

Joe K. Gerald, MD, PhD, Roni Grad, MD, William C. Bailey, MD, Lynn B
Prediction of the incidence, recurrence, and persistence of atopic dermatitis in adolescence: A prospective cohort study  Astrid S. Peters, MD, MSc, Jessica.

Prediction of the incidence, recurrence, and persistence of atopic dermatitis in adolescence: A prospective cohort study  Astrid S. Peters, MD, MSc, Jessica.

Similar presentations

Ads by Google