Presentation is loading. Please wait.

Presentation is loading. Please wait.

Experimental Bronchiolitis Obliterans Induced by In Vivo HVJ-Liposome–Mediated Endothelin-1 Gene Transfer  Shin-ichi Takeda, MD, Yoshiki Sawa, MD, Masato.

Published byMatthew Lyons Modified over 6 years ago

Similar presentations

Presentation on theme: "Experimental Bronchiolitis Obliterans Induced by In Vivo HVJ-Liposome–Mediated Endothelin-1 Gene Transfer  Shin-ichi Takeda, MD, Yoshiki Sawa, MD, Masato."— Presentation transcript:

1 Experimental Bronchiolitis Obliterans Induced by In Vivo HVJ-Liposome–Mediated Endothelin-1 Gene Transfer  Shin-ichi Takeda, MD, Yoshiki Sawa, MD, Masato Minami, MD, Yasufumi Kaneda, MD, Yoshitaka Fujii, MD, Ryota Shirakura, MD, Masashi Yanagisawa, MD, Hikaru Matsuda, MD  The Annals of Thoracic Surgery  Volume 63, Issue 6, Pages (June 1997) DOI: /S (97)

2 Fig. 1 Rat lung tissues from a Wistar rat 7 days after transtracheal introduction of HVJ liposome with β-galactosidase gene. Bronchial epithelium (A) and alveolar cells and alveolar macrophages (B) were stained blue (X-Gal), indicating β-gal activity. (A, ×200; B, ×400; both before 35% reduction.) The Annals of Thoracic Surgery  , DOI: ( /S (97) )

3 Fig. 2 Photomicrographs showing histologic findings of lung tissue from a Wistar rat 7 days after in vivo transfection of HVJ-liposome with an empty expression cassette. No histopathologic changes were seen in the control animals after introduction. (Hematoxylin and eosin; A, ×100; B, ×200; both before 35% reduction.) The Annals of Thoracic Surgery  , DOI: ( /S (97) )

4 Fig. 3 Photomicrographs show histologic changes of lung tissue from Wistar rat on day 3 after in vivo transfection of HVJ-liposome with prepro-ET-1 gene. Histologic section of the lung parenchyma shows alveolar septal thickening due to the interstitial mononuclear cell infiltrate and hyperplasia of the alveolar lining epithelium. No apparent concentric changes were seen in alveolar ducts and small conducting airways. (Hematoxylin and eosin; A, ×100; B, ×200; both before 35% reduction.) The Annals of Thoracic Surgery  , DOI: ( /S (97) )

5 Fig. 4 Photomicrographs showing histologic changes of lung tissue from a Wistar rat on day 7 after in vivo transfection of HVJ-liposome with prepro-ET-1 gene. Hyperplastic connective tissue plaques were seen in the alveolar duct and small conducting airway, identical to human bronchiolitis obliterans (A). Detail of an intrabronchiolar polyp of hyperplastic loose connective tissue (B). (Hematoxylin and eosin; A, ×100; B, ×200; both before 35% reduction.) The Annals of Thoracic Surgery  , DOI: ( /S (97) )

6 Fig. 5 Photomicrographs showing immunohistochemical study of lung tissue from a Wistar rat 7 days after in vivo transfection of HVJ-liposome with prepro-ET-1 gene (A) and HVJ-liposome with an empty expression cassette (B). Strong ET-1–like immunoactivity is seen in airway epithelium or vascular endothelium associated with severe morphologic changes. (Immunohistochemistry; ×100 before 35% reduction.) The Annals of Thoracic Surgery  , DOI: ( /S (97) )

Download ppt "Experimental Bronchiolitis Obliterans Induced by In Vivo HVJ-Liposome–Mediated Endothelin-1 Gene Transfer  Shin-ichi Takeda, MD, Yoshiki Sawa, MD, Masato."

Similar presentations

Adenoviral herpes simplex virus thymidine kinase gene therapy in an orthotopic lung cancer model  Mari Fukunaga, MD, Shinzo Takamori, MD, Akihiro Hayashi,

Adenoviral herpes simplex virus thymidine kinase gene therapy in an orthotopic lung cancer model  Mari Fukunaga, MD, Shinzo Takamori, MD, Akihiro Hayashi,
David D. Yuh, MD, Kimberly L. Gandy, MD, Bruce A

David D. Yuh, MD, Kimberly L. Gandy, MD, Bruce A
Atrial natriuretic peptide gene transfection with a novel envelope vector system ameliorates pulmonary hypertension in rats  Yoshihide Mitani, MD, PhD,

Atrial natriuretic peptide gene transfection with a novel envelope vector system ameliorates pulmonary hypertension in rats  Yoshihide Mitani, MD, PhD,
Extralobar Pulmonary Sequestration Presenting as a Mediastinal Malignancy  Jeffrey M Sippel, MD, Pasala S Ravichandran, MD, Ruza Antonovic, MD, William.

Extralobar Pulmonary Sequestration Presenting as a Mediastinal Malignancy  Jeffrey M Sippel, MD, Pasala S Ravichandran, MD, Ruza Antonovic, MD, William.
Gene Transfer Into the Donor Heart During Cold Preservation for Heart Transplantation  Satoshi Gojo, Kazuo Niwaya, Shigeki Taniguchi, Kazuhiko Nishizaki,

Gene Transfer Into the Donor Heart During Cold Preservation for Heart Transplantation  Satoshi Gojo, Kazuo Niwaya, Shigeki Taniguchi, Kazuhiko Nishizaki,
Optimal time for cardiomyocyte transplantation to maximize myocardial function after left ventricular injury  Ren-Ke Li, MD, PhD, Donald A.G. Mickle,

Optimal time for cardiomyocyte transplantation to maximize myocardial function after left ventricular injury  Ren-Ke Li, MD, PhD, Donald A.G. Mickle,
Volume 24, Issue 2, Pages (June 2017)

Volume 24, Issue 2, Pages (June 2017)
Gene transfer to vein graft wall by HVJ–liposome method: time course and localization of gene expression  Hong-zhi Bai, MD, Yoshiki Sawa, MD, Wei-da Zhang,

Gene transfer to vein graft wall by HVJ–liposome method: time course and localization of gene expression  Hong-zhi Bai, MD, Yoshiki Sawa, MD, Wei-da Zhang,
Time course and cellular localization of inducible nitric oxide synthases expression during cardiac allograft rejection  Neil K Worrall, MD, Thomas P.

Time course and cellular localization of inducible nitric oxide synthases expression during cardiac allograft rejection  Neil K Worrall, MD, Thomas P.
Inhibitory Effects of Calcitonin Gene-Related Peptides on Experimental Vein Graft Disease  Xiaoning Zhang, MD, Jian Zhuang, MD, Hongsui Wu, MB, Zhihong.

Inhibitory Effects of Calcitonin Gene-Related Peptides on Experimental Vein Graft Disease  Xiaoning Zhang, MD, Jian Zhuang, MD, Hongsui Wu, MB, Zhihong.
Pulmonary vascular changes induced by congenital obstruction of pulmonary venous return  Masato Endo, MD, Shigeo Yamaki, MD, Mikio Ohmi, MD, Koichi Tabayashi,

Pulmonary vascular changes induced by congenital obstruction of pulmonary venous return  Masato Endo, MD, Shigeo Yamaki, MD, Mikio Ohmi, MD, Koichi Tabayashi,
Bronchiolitis Obliterans Organizing Pneumonia Due to Titanium Nanoparticles in Paint  Tong-Hong Cheng, MD, Fu-Chang Ko, MD, Junn-Liang Chang, MD, Kuo-An.

Bronchiolitis Obliterans Organizing Pneumonia Due to Titanium Nanoparticles in Paint  Tong-Hong Cheng, MD, Fu-Chang Ko, MD, Junn-Liang Chang, MD, Kuo-An.
Dhruva K. Mishra, PhD, Michael J. Thrall, MD, Brandi N

Dhruva K. Mishra, PhD, Michael J. Thrall, MD, Brandi N
Combined Strategy Using Myoblasts and Hepatocyte Growth Factor in Dilated Cardiomyopathic Hamsters  Haruhiko Kondoh, MD, Yoshiki Sawa, MD, Norihide Fukushima,

Combined Strategy Using Myoblasts and Hepatocyte Growth Factor in Dilated Cardiomyopathic Hamsters  Haruhiko Kondoh, MD, Yoshiki Sawa, MD, Norihide Fukushima,
Short-Course Rapamycin Treatment Preserves Airway Epithelium and Protects Against Bronchiolitis Obliterans  Jacob R. Gillen, MD, Yunge Zhao, MD, PhD,

Short-Course Rapamycin Treatment Preserves Airway Epithelium and Protects Against Bronchiolitis Obliterans  Jacob R. Gillen, MD, Yunge Zhao, MD, PhD,
Adenoviral herpes simplex virus thymidine kinase gene therapy in an orthotopic lung cancer model  Mari Fukunaga, MD, Shinzo Takamori, MD, Akihiro Hayashi,

Adenoviral herpes simplex virus thymidine kinase gene therapy in an orthotopic lung cancer model  Mari Fukunaga, MD, Shinzo Takamori, MD, Akihiro Hayashi,
Surgical Lung Biopsy in Transplant Patients With Diffuse Lung Disease: How Much Worse When the Lung Is the Graft?  Alejandro Bertolotti, MD, Sebastián.

Surgical Lung Biopsy in Transplant Patients With Diffuse Lung Disease: How Much Worse When the Lung Is the Graft?  Alejandro Bertolotti, MD, Sebastián.
Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 
Giant Costal Osteochondroma in a Man With Multiple Exostoses

Giant Costal Osteochondroma in a Man With Multiple Exostoses
Successful resection of an epicardial cyst

Successful resection of an epicardial cyst

Similar presentations

Ads by Google