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Short-Course Rapamycin Treatment Preserves Airway Epithelium and Protects Against Bronchiolitis Obliterans  Jacob R. Gillen, MD, Yunge Zhao, MD, PhD,

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Presentation on theme: "Short-Course Rapamycin Treatment Preserves Airway Epithelium and Protects Against Bronchiolitis Obliterans  Jacob R. Gillen, MD, Yunge Zhao, MD, PhD,"— Presentation transcript:

1 Short-Course Rapamycin Treatment Preserves Airway Epithelium and Protects Against Bronchiolitis Obliterans  Jacob R. Gillen, MD, Yunge Zhao, MD, PhD, David A. Harris, BS, Damien J. LaPar, MD, MSc, Irving L. Kron, MD, Christine L. Lau, MD, MBA  The Annals of Thoracic Surgery  Volume 96, Issue 2, Pages (August 2013) DOI: /j.athoracsur Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 Loss of tracheal luminal epithelium 14 days after transplantation. Early rapamycin treatment shows protection against epithelial loss. n = 8 tracheas per group. Data shown are mean percentage of epithelial loss for each group. (DMSO = dimethyl sulfoxide.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 Representative hematoxylin and eosin stains of tracheal allografts 14 days after transplantation. Treatment with rapamycin shows preservation of tracheal luminal epithelium (arrows) (×40 image). By contrast, control animals treated with dimethyl sulfoxide (DMSO) show complete loss of tracheal epithelium (arrows) (×40 image). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 Representative immunohistochemical staining of tracheal allografts 14 days after transplantation. p63 and CK14 both stain for basal epithelial cells. Rapamycin treatment prevents loss of this epithelial cell population in comparison with dimethyl sulfoxide (DMSO) control animals. Magnification is ×40. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Loss of tracheal luminal epithelium 28 days after transplantation. Rapamycin treatment shows protection against epithelial loss, with treatment from days 1 through 14 as the most effective regimen. n = 8 tracheas per group. Data shown are mean percentage of epithelial loss for each group. (DMSO = dimethyl sulfoxide.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

6 Fig 5 Tracheal luminal obliteration 28 days after transplantation. Rapamycin treatment shows protection against luminal obliteration, with treatment from days 1 through 14 and days 3 through 7 as the most effective regimens. n = 8 tracheas per group. Data shown are mean percent luminal obliteration for each group. (DMSO = dimethyl sulfoxide.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

7 Fig 6 Representative hematoxoylin and eosin stains of trachea 28 days after transplantation. Treatment with rapamycin shows no luminal obliteration (×4 image) and preservation of luminal epithelium (×20 image). By contrast, dimethyl sulfoxide (DMSO) control animals show complete luminal fibroobliteration (×4 image) and complete loss of tracheal epithelium (×20 image). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2013 The Society of Thoracic Surgeons Terms and Conditions

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