Gel pictures of cloning lab

Section1 Gel1 3 kb 5 kb 1.6 kb M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)

Section1 Gel2 M 1 2 Group1Group2 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette) 5 Kb 3 Kb 1.6 Kb

Section1 Gel3 3 kb 5 kb 1.6 kb M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)

Section1 “control gel” M M: DNA 1Kb ladder; 1: Purified cut vector (it did not work. But the band should be in the position of red bar); 2:Cut vector (not purified); 3:uncut vector (labeled as PBS); 4: Purified insert (CAT cassette); 5: Cut insert plasmid (not purified); 6: uncut insert plasmid (labeled as CAT) 5 kb 3 kb 1.6 kb

Section2 Gel1 M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT) 5 Kb 3 Kb 1.6 Kb

Section2 Gel2 M 1 2 Group1Group2Group3 M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT) 5 Kb 3 Kb 1.6 Kb

Section2 Gel3 M 1 2 Group1 M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT) 5 Kb 3 Kb 1.6 Kb